Polygalacturonic acid from orange (Sigma P3889, Germany), was used as a substrate for the determination of endo-polygalacturonase activity. Folin ciocalteu′s phenol reagent (2 N), (Sigma-Aldrich F9252, Germany) was used for the estimation of protein content. Bovine serum albumin, (Sigma A2153, Germany), used for the preparation of standard curve for calculation of protein content.
Polygalacturonic acid from orange
Manufactured by Merck Group
Sourced in Germany, Spain, United States
Polygalacturonic acid from orange is a naturally-derived compound extracted from orange peels. It functions as a structural component in plant cell walls.
Automatically generated - may contain errors
Lab products found in correlation
Bovine serum albumin (bsa), by Merck Group (2 mentions)
Folin ciocalteu s phenol reagent 2 n, by Merck Group (2 mentions)
Dinitrosalicylic acid, by Merck Group (1 mentions)
Dialysis tubing cellulose membrane, by Merck Group (1 mentions)
Chitosan, by Merck Group (1 mentions)
Pectin, by Merck Group (1 mentions)
Sodium chloride (nacl), by Merck Group (1 mentions)
Sodium hydroxide (naoh), by Merck Group (1 mentions)
Hydrochloric acid (hcl), by Carlo Erba (1 mentions)
Acetic acid, by Carlo Erba (1 mentions)
3 protocols using polygalacturonic acid from orange
1
Polygalacturonase Activity Assay
2
Protein Quantification and Enzyme Activity Assays
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Dialysis tubing cellulose membrane (flat width 4.3 cm), (Sigma D9527, Germany). Folin ciocalteu’s phenol reagent (2 N) (Sigma-Aldrich F9252, USA) was used to estimate protein content. Bovine serum albumin (Sigma A2153, USA) was used to prepare the standard curve for calculating protein content. Sephadex G-200 (Pharmacia 17-0080-01, Sweden) was used for gel filtration chromatography. Dinitrosalicylic acid (Sigma D0550, USA) was used as a reagent to determine exo-PG activity. Polygalacturonic acid from orange (Sigma P3889, Spain) was used as a substrate to determine exo-PG activity. D-Galacturonic acid (Fluka 48280, Slovakia) was used to prepare the standard curve for calculating reducing sugar.
3
Chitosan-Polygalacturonic Acid Hydrogel Characterization
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Low molecular weight (LMW) chitosan (MW 150 kDa; [η] = 2.37 dL/g; degree of acetylation DA = 13%), polygalacturonic acid from orange, MW 18 kDa, degree of esterification (DE) 10,6% (Cesàro et al., 1982) , pectin from citrus fruit (MW 17 kDa, DE 22%), pectin from apple (MW 30-100 kDa, degree of esterification 71%), bovine serum albumin (BSA), albumin from chicken egg albumen (OVA), technical grade pentasodium tripolyphosphate (TPP), sodium acetate, sodium hydroxide, and sodium chloride were all purchased from Sigma-Aldrich Co. (St. Louis, Missouri, USA). Acetic acid and hydrochloric acid were obtained from Carlo Erba Reagents (Carlo Erba, Milan, Italy). All other chemicals were of the highest purity grade commercially available and used without further purification.
The commercial chitosan sample was purified and characterized as reported elsewhere (Donati et al., 2005) . The intrinsic viscosity of chitosan was measured by employing a Schott-Geräte AVS/G automatic apparatus and an Ubbelohde type viscometer (in acetate buffer 0.25 M, pH 4.7), as reported in the previous paper (Rampino et al., 2013) .
The commercial chitosan sample was purified and characterized as reported elsewhere (Donati et al., 2005) . The intrinsic viscosity of chitosan was measured by employing a Schott-Geräte AVS/G automatic apparatus and an Ubbelohde type viscometer (in acetate buffer 0.25 M, pH 4.7), as reported in the previous paper (Rampino et al., 2013) .
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