Data were collected in the beamline 15A using an Rayonix MX300HE CCD Area Detector at NSRRC. Data sets were indexed, integrated, and scaled using HKL-2000 [78 (link)] and XDS [79 (link)]. The initial phase, density modification, and model building were performed using the AutoSol program in the PHENIX [80 (link)]. The iterative model building and structure refinement were performed using Refmac in the CCP4 software suite (version v7.1.008) [81 (link)] and phenix.refine in the PHENIX software suite (Phenix1.19.1-4122) [82 (link)]. The initial phase of SaSsbA complexed with 5-FU was determined through the molecular replacement software Phaser MR (Phenix1.19.1-4122) [83 (link)] by using SaSsbA (PDB ID 5XGT) as a search model. The correctness of the stereochemistry of the models was verified using MolProbity [84 (link)].
Mx300he ccd area detector
Manufactured by Rayonix
The MX300HE CCD Area Detector from Rayonix is a high-efficiency, large-area X-ray detector designed for macromolecular crystallography and other X-ray diffraction applications. The detector features a 300 mm active area, high quantum efficiency, and low noise characteristics.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using mx300he ccd area detector
1
Structural Determination of SaSsbA-5FU Complex
2
Structural Determination of SaSsbA-5FU Complex
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Data were collected in the beamline 15A using an Rayonix MX300HE CCD Area Detector at NSRRC. Data sets were indexed, integrated, and scaled using HKL-2000 [78 (link)] and XDS [79 (link)]. The initial phase, density modification, and model building were performed using the AutoSol program in the PHENIX [80 (link)]. The iterative model building and structure refinement were performed using Refmac in the CCP4 software suite (version v7.1.008) [81 (link)] and phenix.refine in the PHENIX software suite (Phenix1.19.1-4122) [82 (link)]. The initial phase of SaSsbA complexed with 5-FU was determined through the molecular replacement software Phaser MR (Phenix1.19.1-4122) [83 (link)] by using SaSsbA (PDB ID 5XGT) as a search model. The correctness of the stereochemistry of the models was verified using MolProbity [84 (link)].
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!