Mitoscreen jc 1 staining kit

Flow cytometric analysis of apoptosis was carried out using the FITC Annexin V Apoptosis Detection Kit I (BD Biosciences, Bedford, MA, USA), and was performed and presented according to the described protocol. Briefly, cells were dissociated with trypsin and resuspended at 1×10⁶ cells/ml in binding buffer with 50 µl/ml FITC Annexin V and 50 µl/ml propidium iodide (PI). The cells were subsequently incubated for 15 min at room temperature, and then were analyzed using a Gallios flow cytometer (Beckman Coulter, Inc. Brea, CA, USA). The cell inner mitochondrial membrane potential (Δψm) was detected by flow cytometry using MitoScreen JC-1 staining kit (BD Biosciences), and was carried out and presented according to the described protocol. Briefly, cells were dissociated with trypsin and resuspended at 1×10⁶ cells/ml in assay buffer, and then incubated at 37°C for 15 min with 10 µl/ml JC-1. Before being analyzed by flow cytometry, the cells were washed twice using assay buffer. Flow cytometric data were analyzed using FlowJo 7.6 software (TreeStar, Inc., Ashland, OR, USA).

Flow cytometric analysis of apoptosis was performed using the fluorescein isothiocyanate (FITC) Annexin V Apoptosis Detection kit I (BD Biosciences, San Jose, CA, USA) and performed as previously described (27 (link)). Briefly, cells were dissociated with trypsin and resuspended at 1×10⁶ cells/ml in binding buffer with 50 µl/ml FITC Annexin V and 50 µl/ml propidium iodide (PI). Cells were subsequently incubated for 15 min at room temperature and analyzed using a Gallios flow cytometer (Beckman Coulter, Inc., Brea, CA, USA). The cell's inner mitochondrial membrane potential (Δψm) was detected by flow cytometry using a MitoScreen JC-1 staining kit (BD Biosciences) following a previously described protocol (27 (link)). Briefly, cells were dissociated with trypsin, resuspended at 1×10⁶ cells/ml in assay buffer and then incubated at 37°C for 15 min with 10 µl/ml JC-1. Cells were washed twice with the assay buffer prior to analysis with the flow cytometer. Flow cytometric data were analyzed using FlowJo 7.6 software (FlowJo LLC, Ashland, OR, USA).

Flow cytometric analysis of apoptosis was done using the fluorescein isothiocyanate (FITC) Annexin V Apoptosis Detection Kit I (BD, USA) and was performed as described by the protocol. Briefly, cells were dissociated with trypsin and resuspended at 1 × 10⁶ cells/mL in binding buffer with 50 μL/mL FITC Annexin V and 50 μL/mL propidium iodide (PI). The cells were subsequently incubated for 15 min at room temperature and then analyzed with a Gallios flow cytometer (Beckman Coulter, USA). The cells’ inner mitochondrial membrane potential (Δψm) was detected by flow cytometry using the MitoScreen JC-1 staining kit (BD) and was performed as described in the protocol. Briefly, cells were dissociated with trypsin, resuspended at 1 × 10⁶ cells/mL in assay buffer, and then incubated at 37°C for 15 min with 10 μl/mL JC-1. Before being analyzed by flow cytometer, cells were washed twice with assay buffer. Flow cytometry data were analyzed using FlowJo 7.6 software (Tree Star, USA).

Flow cytometric analysis of apoptosis was carried out using the FITC Annexin V Apoptosis Detection kit I (BD Biosciences, San Jose, CA, USA), and was presented as the protocol described below. Briefly, the cells were dissociated with trypsin and resuspended at 1×10⁶ cells/ml in binding buffer with 50 µl/ml FITC Annexin V and 50 µl/ml PI. The cells were subsequently incubated for 15 min at room temperature, and were then were analyzed using a Gallios flow cytometer (Beckman Coulter, Atlanta, GA, USA). The cell inner mitochondrial membrane potential (Δψm) was detected by flow cytometry using the MitoScreen JC-1 staining kit (BD Biosciences), and was presented as the protocol described below. Briefly, the cells were dissociated with trypsin and resuspended at 1×10⁶ cells/ml in Assay Buffer, and then incubated at 37°C for 15 min with 10 µl/ml JC-1. Before analyzed by flow cytometer, cells were washed twice by Assay Buffer. Flow cytometry data were analyzed using FlowJo 7.6 software (TreeStar Inc., Ashland, OR, USA).