Log In Sign up
The largest database of trusted experimental protocols

Annexin 5 propidium iodide detection kit

Manufactured by Beyotime
Visit Supplier

The Annexin V/propidium iodide (PI) detection kit is a laboratory tool used to identify and quantify apoptotic and necrotic cells. The kit utilizes Annexin V, a protein that binds to phosphatidylserine, and propidium iodide, a dye that stains DNA, to distinguish between different cell death processes.

Automatically generated - may contain errors

Lab products found in correlation

Close spelling variants of this product

FITC Annexin V Apoptosis Detection Kit with PI (propidium iodide), FITC-conjugated Annexin V (Annexin V-FITC)/propidium iodide (PI) Apoptosis Detection kit FITC-Annexin-V and propidium iodide (PI) Apoptosis Detection Kit Annexin V/propidium iodide (PI) apoptosis detection kit Annexin V-uorescein isothiocyanate (FITC)/propidium iodide (PI) Apoptosis Detection Kit Annexin V-FITC/propidium iodide (PI) apoptosis detection kit Annexin V-fluorescein isothiocyanate/propidium iodide Apoptosis Detection Kit Annexin V-fluorescein isothiocyanate (FITC) and propidium iodide (PI) apoptosis detection kit Annexin V-FITC/ Propidium Iodide (PI) detection kit Annexin V-fluorescein isothiocyanate (FITC)/propidium iodide (PI) Apoptosis Detection Kit

3 protocols using annexin 5 propidium iodide detection kit

1

Quantifying Apoptosis by Annexin V/PI

Check if the same lab product or an alternative is used in the 5 most similar protocols
Cell apoptosis was quantified using the Annexin V/propidium iodide (PI) detection kit (Beyotime, Shanghai, China) and analyzed by flow cytometry. Cells (2 × 105/well) were plated in 6-well dishes and treated with 10 μM curcumin or no curcumin exposed to 10 μM KU55933 or no KU55933. After treatment, collected cells were incubated in 400 μl binding buffer with 5 μl Annexin V-FITC and 5 μl PI in dark for 15 min at room temperature.
Hu A., Huang J.J., Li R.L., Lu Z.Y., Duan J.L., Xu W.H., Chen X.P, & Fan J.P. (2015). Curcumin as therapeutics for the treatment of head and neck squamous cell carcinoma by activating SIRT1. Scientific Reports, 5, 13429.
+ Open protocol
+ Expand
2

Quantifying Cell Apoptosis by Flow Cytometry

Check if the same lab product or an alternative is used in the 5 most similar protocols
Cell apoptosis was quantified using the Annexin V/propidium iodide (PI) detection kit (Beyotime, Shanghai, China) and analyzed by flow cytometry. Cells (2 × 105/well) were plated in 6-well dishes and treated with 10 μM CUR or no CUR exposed to 10 μM KU55933 or no KU55933. After treatment, collected cells were incubated in 400 μl binding buffer with 5 μl Annexin V-FITC and 5 μl PI in dark for 15 min at room temperature.
Hu A., Huang J.J., Zhang J.F., Dai W.J., Li R.L., Lu Z.Y., Duan J.L., Li J.P., Chen X.P., Fan J.P., Xu W.H, & Zheng H.L. (2017). Curcumin induces G2/M cell cycle arrest and apoptosis of head and neck squamous cell carcinoma in vitro and in vivo through ATM/Chk2/p53-dependent pathway. Oncotarget, 8(31), 50747-50760.
+ Open protocol
+ Expand
3

Annexin V-PI Apoptosis Assay in HUVECs

Check if the same lab product or an alternative is used in the 5 most similar protocols
Flow cytometry was used to detect the apoptosis level of HUVECs using annexin V-propidium iodide (PI) detection kit (Beyotime, C1062S). The HUVECs were collected, and digested with trypsin, then incubated with FITC-conjugated annexin and PI according to protocol. The fluorescence of HUVECs was analyzed with a flow cytometer (Beckman Gallious). PI-negative and ANXA5-positive cells were considered as early apoptotic cells, PI-positive and ANXA5-positive cells were considered as advanced apoptotic cells. The results were quantified by FACScan (Beckman Gallious) and analyzed with FlowJo7.6 software.
Zhang S., Zhao J., Wu M., Zhou Y., Wu X., Du A., Tao Y., Huang S., Cai S., Zhou M., Wei T., Zhang Y., Xie L., Wu Y, & Xiao J. (2023). Over-activation of TRPM2 ion channel accelerates blood-spinal cord barrier destruction in diabetes combined with spinal cord injury rat. International Journal of Biological Sciences, 19(8), 2475-2494.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!