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2 protocols using anti myc hrp

1

Investigating Viral RNA Sensing Pathways

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Sendai virus was purchased from The American Type Culture Collection (ATCC, Manassas, VA). Reovirus was purchased from Advanced Biotechnologies, Inc. (Columbia, MD). Poly I:C, poly dG:dC and biotin-labeled Poly I:C were purchased from InvivoGen (San Diego, CA). Lipofectamine 2000 was purchased from Invitrogen (Carlsbad, CA). DHX15 antibody was purchased from Abcam (Cambridge, MA) and used for immunoprecipitation and immunoblotting. The following antibodies were used for immunoblotting: anti-IRF3 (Santa Cruz, Dallas, TX); anti-DDX21 (Novus Biologicals, Littleton, CO); anti-MAVS, anti-STING, anti-Erk1/2, anti-p38, anti-p65, anti-phospho-Erk1/2, anti-phospho-p38, anti-phospho-p65 and anti-phospho-IRF3 (Cell Signaling, Danvers, MA); anti-DHX41, anti-GAPDH-HRP, anti-Flag-HRP, Anti-HA-HRP and anti-Myc-HRP (Sigma, St. Louis, MO). Anti-HA and anti-Myc beads were purchased from Sigma. Protein A/G beads and NeutAvidin beads were purchased from Thermo Scientific (Rockford, IL).
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2

Western Blot Analysis of Signaling Proteins

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Western blot analysis was performed as described [5 (link), 18 (link)]. The primary antibodies used for Western blot analysis were anti-phospho-serine (Chemicon), β-actin (Santa Cruz), anti-Flag-HRP (Sigma), anti-HA-HRP (Sigma), anti-myc-HRP (Sigma), anti-SPDEF (Santa Cruz), and anti-CDK11p58 (Santa Cruz).
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