Synapsin slides were blocked for 60 min in 5% (w/v) skimmed milk powder 0.05% Tween20 PBS pH 7.4. Afterward, slides were incubated for 30 min with positive and negative serum as determined with cell-based assays (1:1000 dilution). Washing steps were carried out three times using 0.05% Tween20 PBS. IgG antibodies were detected using goat-anti-human secondary antibodies (Thermo Fisher, #31410, 1:2500). Chemiluminescence signal was detected with an Azure system c400 using SuperSignal West Femto maximum sensitive substrate (Thermo Scientific, Schwerte, Germany). Microarray binding intensities were quantified using the software-tool MARTin (https://github.com/scitequest/martin ) and normalized to the most prominent binder. Heat-maps were generated with OriginPro 2021 9.8.0.200 (OriginLab, Northampton, MA). Human synapsin-I 3D model was extracted from AlphaFold (DeepMind Technologies, London, UK) (Jumper et al., 2021 (link)) database and rendered using Pymol 2.4 (Schrödinger Inc, New York, U.S.).
Originpro 2021 9
Manufactured by OriginLab
Sourced in United States
OriginPro 2021 9.8.0.200 is a data analysis and graphing software developed by OriginLab. It provides tools for data manipulation, advanced data analysis, and high-quality 2D and 3D data visualization.
Automatically generated - may contain errors
Lab products found in correlation
4 protocols using originpro 2021 9
1
Synapsin Antibody Binding Assessment
2
Optimized Analytical Procedures via OriginPro
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Analysis was performed using OriginPro 2021 9.8.0.200 (OriginLab, Northampton, MA) and error bars represent mean ± SD unless otherwise noted. Replicates are technical, representing independent measurements. Number of repetitions varied depending on methodology and is defined in the figure legends.
3
Multivariate Statistical Analysis of Data
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Statistical analysis was performed using the TIBCO Statistica® 13.3.0. and OriginPro 2021 9.8.0.200 with a significance level (p) of 0.05. The parametric data analysis was carried out using analysis of variance (ANOVA) based on a pairwise means comparison by using Tukey statistical tests.
4
Skin Irritation Evaluation Protocol
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All experimental results were analyzed using Origin Pro 20219.8.0.200 (Origin Lab, Northampton, MA, USA), Image Tool software and Sigma Plot for Windows Version 12.3 (Sigma Plot Software, Palo Alto, CA, USA). All data were expressed as mean ± standard deviations (SD) of the indicated number of experiments. Statistical analysis was performed using ANOVA followed by Student's t-test (p < 0.05) for independent variables. Erythema/edema scores were evaluated by the Draize dermal irritation scoring system (DDISS). For all tests, a P value < 0.05 was considered statistically signi cant.
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