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Anti myc c3956

Manufactured by Merck Group

Anti-Myc (C3956) is a laboratory reagent produced by Merck Group. It is a monoclonal antibody that recognizes the Myc protein, which is a common protein tag used in molecular biology experiments. The core function of Anti-Myc (C3956) is to detect and identify proteins that are tagged with the Myc epitope.

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2 protocols using anti myc c3956

1

Reagent Acquisition and Antibody Details

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ABC was purchased from Carbosynth (NA10019). CPT-11 was purchased from TopoGEN (TG4110), cisplatin was purchased from Nihon-kayaku (Randa), etoposide was purchased from TREVIGEN (4886–400–01), and adriamycin was purchased from Kyowa Hakko Kirin (Adriacin). These chemicals were dissolved in 100% dimethyl sulfoxide (Nacalai Tesque). Antibodies used were as follows: anti-FLAG M2 (F9291, Sigma-Aldrich), anti-Myc (C3956, Sigma-Aldrich), anti-NRF-1(H-300, sc-33771, Santa Cruz), anti-TDP1 (ab4166, Abcam), and anti–β-actin (AC-15, A5441, Sigma-Aldrich). Anti-Tax (Lt-4) antibody was as described previously46 (link).
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2

Antibody and Reagent Sourcing for Cell Signaling

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Anti-HA (3724), anti-GSDMD (39754) and anti-Acetylated Lysine (9441) antibodies were purchased from Cell Signaling Technology. Anti-GAPDH antibody, HRP-labeled goat anti-mouse IgG and goat anti-rabbit IgG were from Hangzhou Fudebio. Anti-Flag M2 (F1804), anti-Flag M2 Magnetic Beads (10004D), anti-Myc (C3956) antibodies and LPS (O111:B4, L2630) were purchased from Sigma-Aldrich. Anti-FLAG antibody (rabbit source), Goat pAb to MS IgG (Chromeo 555, ab60316), Dnk pAb to Rb IgG (Alexa Fluor 647, ab150075) fluorescent secondary antibodies were obtained from Abcam. Nigericin, Pam3CSK4 (TLRL-PMS), poly(dA:dT) (TLRL-PATN) and flagellin (TLRL-EPSTFLA) were purchased from InvivoGen. ELISA kits for analysis of human IL-1β, mouse IL-1β, mouse IL-6 and mouse TNF-α were purchased from MultiSciences. CytoTox 96 LDH-release assay kit (G1780) was from Promega. Efficient eukaryotic transfection reagent VigoFect was purchased from Vigorous Biotechnology (Beijing), Lipofectamine 2000 transfection reagent was obtained from Invitrogen while Lipo8000 transfection reagent was obtained from Beyotime Biotechnology. Trichostain A (HY-15144), Nicotinamide (HY-B0150), LMK-235(HY-18998) and okadaic acid (HY-N6785) were purchased from MCE.
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