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Bigdye ver 1.1 terminator kit

Manufactured by Thermo Fisher Scientific
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The BigDye ver. 1.1 Terminator kit is a reagent kit designed for DNA sequencing applications. The kit contains the necessary components, including dye-labeled terminators, to perform DNA sequencing reactions. The kit is intended for use with compatible DNA sequencing instruments and analysis software.

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Lab products found in correlation

Abi 310 genetic analyzer, by Thermo Fisher Scientific (2 mentions)

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BigDye Terminator kit (210 citations) BigDye Terminator (138 citations)

2 protocols using bigdye ver 1.1 terminator kit

1

Genetic Sequencing of TERT Promoter

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Standard PCR was carried out for genetic sequencing to identify TERT mutation. Briefly, a fragment of TERT promoter was amplified by PCR on genomic DNA using primers previously described 31 (link). Thirty nanograms of genomic DNA was used in PCR (initial activation step at 95°C for 3 min, forty cycles of 95°C denaturation for 30 sec, 62°C annealing for 1 min, 72°C elongation for 2,5 min, and a final elongation step at 72°C for 7 min). TERT promoter sequences were generated by direct sequencing with an ABI 310 Genetic Analyzer (Applied Biosystems) utilizing BigDye ver. 1.1 terminator kit (Applied Biosystems).
Caria P., Pillai R., Dettori T., Frau D.V., Zavattari P., Riva G., Romano G., Pani F., Bentivegna A., Giovannoni R., Pagni F., Sogos V, & Vanni R. (2017). Thyrospheres from B-CPAP Cell Line with BRAF and TERT Promoter Mutations have Different Functional and Molecular Features than Parental Cells. Journal of Cancer, 8(9), 1629-1639.
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2

BRAF Gene Exon 15 Mutation Screening

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Exon 15 of the BRAF gene was amplified by PCR as previously described 30 (link). Confirmation of mutation was performed by direct sequencing with an ABI 310 Genetic Analyzer (Applied Biosystems, Milan, Italy) utilizing BigDye ver. 1.1 terminator kit (Applied Biosystems) and specific amplification primers for exon 15 (Life Technologies).
Caria P., Pillai R., Dettori T., Frau D.V., Zavattari P., Riva G., Romano G., Pani F., Bentivegna A., Giovannoni R., Pagni F., Sogos V, & Vanni R. (2017). Thyrospheres from B-CPAP Cell Line with BRAF and TERT Promoter Mutations have Different Functional and Molecular Features than Parental Cells. Journal of Cancer, 8(9), 1629-1639.
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