Nutrient media were prepared on the basis of commercial Murashige and Skoog Basal Salt Mixture (MS) (MS5524, Sigma–Aldrich, Saint Louis, MO, USA). Media for co-cultivation, regeneration, shoot elongation, and rooting were prepared by supplementing the base MS5524 medium with the components listed in Table 1 .
The freshly prepared medium was autoclaved at 121 °C and 220 kPa for 15 min and dispensed in 50 mL portions into sterile polycarbonate GA-7-3 culture vessels (Sigma-Aldrich, Saint Louis, MO, USA) and in 10 mL portions into 150 × 25 mm glass tissue-culture tubes (Z681784, Sigma-Aldrich, Saint Louis, MO, USA) with plastic vented closures. Kanamycin sulfate (A1493, AppliChem, Barcelona, Spain) and cefotaxime (Lekko, Volginskiy setl., Russia) were added to the air-cooled autoclaved medium (55 °C) to prevent thermal degradation of the antibiotic.
The freshly prepared medium was autoclaved at 121 °C and 220 kPa for 15 min and dispensed in 50 mL portions into sterile polycarbonate GA-7-3 culture vessels (Sigma-Aldrich, Saint Louis, MO, USA) and in 10 mL portions into 150 × 25 mm glass tissue-culture tubes (Z681784, Sigma-Aldrich, Saint Louis, MO, USA) with plastic vented closures. Kanamycin sulfate (A1493, AppliChem, Barcelona, Spain) and cefotaxime (Lekko, Volginskiy setl., Russia) were added to the air-cooled autoclaved medium (55 °C) to prevent thermal degradation of the antibiotic.