Spectrospin spectrometer

Unless otherwise stated, Aldrich reagents were used as supplied. Uncorrected electrothermal melting points were measured in England. A Buchi rotary evaporator (W. Germany) with a bath temperature below 40 °C evaporated under decreased pressure. A Bruker spectrospin spectrometer (Germany) at the BCSIR Laboratories in Dhaka acquired 400 MHz and 100 MHz ¹H NMR and ¹³C NMR spectra for solutions in deuteriochloroform (CDCl₃) unless specified (internal Me4Si). Thin layer chromatography (t.l.c) on Kieselgel GF254-detected spots by spraying the plates with 1% H₂SO₄ and heating at 150–200 °C until coloration occurred. Column chromatography used silica gel G₆₀.

Materials and instrumentations: Roswell Park Memorial Institute (RPMI) 1640 medium, foetal bovine serum (FBS), penicillin and streptomycin, phosphate‐buffered saline (PBS, pH 7.4) and other tissue culture reagents were purchased from Gibco (Thermo Fisher Scientific, UK). Non‐dried solvents were purchased from Fischer Scientific and used as received. Dichloromethane was dried over molecular sieves. All reactions were performed under standard Schlenk conditions unless otherwise stated. All phosphines were kept under vacuum and handled under constant flow of nitrogen. [(η⁶‐p‐Cymene)Ru(Mnt)] was synthesised according to a previously reported method.^[13] All NMR spectra were recorded on a 400 MHz Bruker Spectrospin spectrometer using 5 mm NMR tubes. Deuterated solvents were purchased from Goss Scientific Instrument. The ¹H and ¹³C NMR chemical shifts were internally referenced to TMS via residual solvent peaks CHCl₃ (δ=7.26 and 77.16 ppm). Coupling constants are in Hz; abbreviations: s, singlet; d, doublet; sept, septuplet; m, multiplet. Cell lines were provided by the Institute of Cancer Therapeutics, University of Bradford, UK. Cells were incubated in a ThermoScientific HERAcell 150 incubator, and observed under a Nikon ECLIPSE TS100 Microscope.