P plcγ1 y783

Manufactured by Cell Signaling Technology

Sourced in United States

P‐PLCγ1 (Y783) is an antibody that detects phosphorylation of PLCγ1 at tyrosine 783. PLCγ1 is an enzyme that plays a role in signal transduction pathways.

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PLCγ1 (37 citations) P-PLCγ1 (11 citations)

2 protocols using p plcγ1 y783

Signaling Pathways in Chondrocyte Apoptosis

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Antibodies used in this study were purchased from the following companies: antibodies against Collagen2 (1 : 1000), Bcl‐2 (1 : 1000), Bax (S757) (1 : 1000), P53 (1 : 1000), RIP1 (1 : 1000), and RIP3 (1 : 1000) were purchased from Abcam Inc. (Cambridge, MA, USA); antibodies targeting PLCγ1 (1 : 1000), p‐PLCγ1 (Y783) (1 : 1000), and caspase3 (1 : 1000) were purchased from Cell Signaling Technology Inc. (Beverly, MA, USA); and antibodies against Aggrecan (1 : 1000) and β‐actin (1 : 40 000) were purchased from Sigma‐Aldrich in China (Shanghai, China), respectively. Inhibitors used in this study (U73122, Z‐VAD, and Nec‐1) were purchased from MedChemExpress (Monmouth Junction, NJ, USA). Cytokine used in this study such as recombinant rat IL‐1β was purchased from PeproTech (Rocky Hill, NJ, USA). Other reagents were of the highest grade commercially available.

Chen X., Chen R., Xu Y, & Xia C. (2020). PLCγ1 inhibition combined with inhibition of apoptosis and necroptosis increases cartilage matrix synthesis in IL‐1β‐treated rat chondrocytes. FEBS Open Bio, 11(2), 435-445.

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Comprehensive Antibody Panel for Cellular Analysis

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The following antibodies were used: Cdx2 (Biogenex, MU392A-UC), CrkL (Santa Cruz Biotechnology, sc-319), Erk1/2 (Cell Signaling, 9102L), Flag2 M2 (Sigma, F1804), Frs2 (H-91; Santa Cruz Biotechnology, sc-8318), Gata4 C-20 (Santa Cruz Biotechnology, sc-1237), Lamin B M20 (Santa Cruz Biotechnology, sc-6217), Nanog (Reprocell, RCAB0002P-F), pAkt (Ser473) (Cell Signaling, 9271), pAkt (Thr308) 244F9 (Cell Signaling, 4056), pErk1/2 (T202/Y2014) (Cell Signaling, 9101), pFrs2 (Tyr196) (Cell Signaling, 3864), pJnk (T183/Y185) (Cell Signaling, 4671S), Plcγ1 (Cell Signaling, 2822), pp38 (T180/Y182) 28B10 (Cell Signaling, 9216), pPlcγ1 (Y783) (Cell Signaling, 2821), Stat3α (Cell Signaling, 8768), and pStat5 (Y694) D4739 (Cell Signaling, 4322). The anti-β-tubulin E7 antibody developed by M. Klymkowsky was obtained from the Developmental Studies Hybridoma Bank developed under the auspices of the National Institute of Child Health and Human Development and maintained by Department of Biology at The University of Iowa.

Brewer J.R., Molotkov A., Mazot P., Hoch R.V, & Soriano P. (2015). Fgfr1 regulates development through the combinatorial use of signaling proteins. Genes & Development, 29(17), 1863-1874.

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