Mglu5

Manufactured by Merck Group

Sourced in Germany

MGlu5 is a laboratory instrument designed for the detection and quantification of metabotropic glutamate receptor 5 (mGlu5) in biological samples. The core function of the MGlu5 is to provide researchers with a reliable and accurate tool for measuring the expression levels of this important receptor, which plays a key role in various neurological and psychiatric processes.

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Lab products found in correlation

Syngap, by Cell Signaling Technology (2 mentions) Homer1b c, by Santa Cruz Biotechnology (2 mentions) Protein a sepharose beads, by GE Healthcare (2 mentions) Calnexin, by Enzo Life Sciences (1 mentions) Ps tp, by Abcam (1 mentions) Pxpsp, by Cell Signaling Technology (1 mentions) P erk1 2, by Cell Signaling Technology (1 mentions) Phosphoserine, by Thermo Fisher Scientific (1 mentions) Erk1 2, by Cell Signaling Technology (1 mentions)

4 protocols using mglu5

Quantifying Synaptic Protein Interactions

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PSD-enriched preparations (as previously described) of different brain
regions (cortex, hippocampus and striatum) from P60 WT and KO mice were
incubated at 4°C overnight with protein A-Sepharose beads (GE Healthcare)
conjugated to 10 μg/ml of Homer1b/c (Santa Cruz cat. H-342) or of PSD-95
(NeuroMab, Ca, USA cat. 75-028 ) or control IgG (10 μg/ml) antibodies.
The beads were then washed three times with RIPA buffer, re-suspended in sample
buffer, warmed at 65°C for 10 minutes and analyzed using SDS-PAGE.
Western blot analysis was performed using mGlu5 (Millipore, cat. AB5675), SynGAP
(Cell Signaling cat. D78B11) andGluN2A (Sigma cat. G9038) primary
antibodies.

Vicidomini C., Ponzoni L., Lim D., Schmeisser M., Reim D., Morello N., Orelanna D., Tozzi A., Durante V., Scalmani P., Mantegazza M., Genazzani A.A., Giustetto M., Sala M., Calabresi P., Boeckers T.M., Sala C, & Verpelli C. (2016). Pharmacological enhancement of mGlu5 receptors rescues behavioral deficits in SHANK3 knock-out mice. Molecular psychiatry, 22(5), 689-702.

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Western Blotting of Synaptic Proteins

Cited 9 times

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Western blotting was performed on whole tissue homogenates from the AcbSh and AcbC (AP +1.18 mm), and CeA and BLA (AP -1.34 mm) (location relative to bregma, as depicted in Paxinos and Franklin, 2004 ) following procedures identical to those described in Lee et al. (2016) (link). The following primary antibodies and concentrations were used: mGlu1 (Synaptic Systems, Göttingen, Germany; 1:1000 dilution), mGlu5 (Millipore, Temecula, CA, United States; 1:1000 dilution), Homer2b (Millipore, Temecula, CA, United States; 1:1000 dilution), and calnexin (Enzo Life Sciences, Farmingdale, NY, United States; 1:1000 dilution) for standardization. Homer2b is a postsynaptic density scaffolding protein that regulates signaling of Group 1 metabotropic glutamate receptors (mGluRs) (Szumlinski et al., 2005 (link)). Together, these proteins were selected for study based on our laboratory’s prior work identifying them as relevant to alcohol-induced neuroplasticity (Szumlinski et al., 2005 (link), 2007 (link), 2008 (link); Cozzoli et al., 2009 (link), 2012 (link), 2014 (link), 2015 (link); Obara et al., 2009 (link); Goulding et al., 2011 (link); Lum et al., 2014 (link); Lee et al., 2015 (link); Quadir et al., 2016 (link)).

Lee K.M., Coehlo M.A., Solton N.R, & Szumlinski K.K. (2017). Negative Affect and Excessive Alcohol Intake Incubate during Protracted Withdrawal from Binge-Drinking in Adolescent, But Not Adult, Mice. Frontiers in Psychology, 8, 1128.

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Quantifying Synaptic Protein Interactions

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Antibody-Based Analysis of mGlu5 Signaling

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Antibodies used in this study include rabbit antibodies against mGlu5 (Millipore), ERK1/2 (Cell Signaling Technology, Danvers, MA), pERK1/2 (phosphorylation at T202/Y204 for pERK1; Cell Signaling), GST (Sigma-Aldrich, St. Louis, MO), pS/TP (Abcam), or PXpSP (Cell Signaling), phosphoserine (Invitrogen), or mouse antibodies against mGlu5 (Abcam, Cambridge, MA), ERK1 (Abcam), ERK1/2 (Cell Signaling), or pTP (Cell Signaling). Pharmacological agents, including (RS)-3,5-dihydroxyphenylglycine (DHPG), 3-methyl-aminothiophene dicarboxylic acid (3-MATIDA) and U0126, were purchased from Tocris Cookson Inc. (Ballwin, MO). All drugs were freshly prepared at the day of experiments.

Jin D.Z., Mao L.M, & Wang J.Q. (2018). The role of extracellular signal-regulated kinases (ERK) in the regulation of mGlu5 receptors in neurons. Journal of molecular neuroscience : MN, 66(4), 629-638.

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