Uptake and distribution of curcumin in body tissues is crucial for its biological activity. The biodistribution of the curcumin-loaded cerasomes was studied in mice, which were divided into groups: (1) control; (2) only curcumin-loaded cerasomes with no PS 80; (3) only CPC with 5% PS 80; (4) 5% PS 80-modified cerasomes with no curcumin in combination with UTMD; (5) CPC with 5% PS 80 in combination with UTMD (group 5 was treated only on the left side for distinction from group 3). The anesthetized mice (n=6 per group at each time point) were transcardially perfusion-fixed with 4% polyformaldehyde (PFA) in PBS. The curcumin content in dissected major organs (brain, heart, liver, spleen, lung and kidney) ex vivo was measured using the IVIS imaging system (Caliper IVIS Spectrum, PerkinElmer) on the basis of the fluorescence intensity from curcumin (ex. = 420 nm, em. = 520 nm), at 0.1, 6, 12, 24 h after i.v. 50 .
Caliper ivis spectrum
Manufactured by PerkinElmer
Sourced in United States
The Caliper IVIS Spectrum is an in vivo imaging system designed for preclinical research. It enables non-invasive visualization and quantification of bioluminescent and fluorescent signals in small animal models.
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7 protocols using caliper ivis spectrum
1
Curcumin Biodistribution in Cerasome Formulations
2
Quantifying Luciferase Expression in Cells
3
Quantitative Analysis of Tumor Growth and Metastasis
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Measurement of PC3-Luc cellular growth and metastasis was carried out utilizing a Caliper IVIS Spectrum imaging system (PerkinElmer, Waltham, MA). Beginning at 24 hours after orthotopic implantation, mice were imaged weekly for 7 weeks (when mice began to display morbidity). Briefly, mice received an intraperotineal injection of luciferin (150 mg/kg) (PerkinElmer, Waltham, MA) and were imaged 10 minutes later under 2.0% isoflurane anesthesia. At the conclusion of week 7, extant mice received a luciferin injection and femurs, lungs, and primary tumors were expediently excised, weighed, and imaged in a 10 cm cell culture plate. Luminescence was quantified as total flux (photons/second) using LivingImage software (PerkinElmer, Waltham, MA).
4
Orthotopic GBM Xenograft in Nude Mice
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Male BALB/c nude mice were obtained from the Vital River (Beijing, China), 1 × 106 U87 cells stably expressing EGFP-luciferase and transfected with sh-HOXC-AS3 or negative control were injected into the frontal lobes of nude mice for GBM orthotopic xenograft tumorigenesis (6 per group). The tumor volumes were measured 7, 14, and 21 days after the implantation using a bioluminescence imaging system (Caliper IVIS Spectrum, PerkinElmer, USA). D-Luciferin potassium salt (Beyotime, China) was injected intraperitoneally before the measurement. After the mice died, their brain tissue was removed and fixed with paraformaldehyde for further experiments.
5
Intracranial GBM Xenograft Model in Mice
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The animal experiments were approved by the Animal Management Rule of the Chinese Ministry of Health (document 55, 2001) and were performed conforming to the approved guidelines and experimental protocols of Nanjing Medical University. U87 cells (1 × 106) stably expressing MCS-firefly luciferase for bioluminescence imaging were transfected with lentivirus expressing control shRNA, shRNA-ANXA2 or shRNA-miR155HG and then were intracranially injected into the frontal lobe of nude mice to generate GBM (n = 10 mice per group). Tumor volumes were measured by luciferase using a bioluminescence imaging system (Caliper IVIS Spectrum, PerkinElmer, Waltham, MA, USA) on days 1, 11, and 21 after implantation. The integrated flux of photons (photons/s) within each region was determined by the Living Images software package (Caliper Life Sciences). Mice were sacrificed when they were in deep coma. Brains were extracted, fixed in 10% formalin and then embedded in paraffin for IHC or frozen at − 80 °C for western blotting or FISH.
6
In Vivo Tracking of CXCR4-MSC Accumulation
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For in vivo characterization of the CXCR4-MSC accumulation, a near-infrared dye 1,1′-dioctadecyl-3,3,3′,3′-tetramethylindotricarbocyanine [DiR, DiIC18(7), MKBio, China] was used as a marker. The collected MSCs or CXCR4-MSCs were added with a basal Dulbecco’s MEM containing 20 μg of DiR near-infrared dye and stained for 15 min. After washing the stained cells three times using PBS, the cells were suspended in a 0.85% NaCl solution to a density of 3 × 108 cells/ml. After the MSC injection, fluorescence imaging of the mice was performed on days 0, 1, 3, and 5 using an IVIS (Caliper IVIS Spectrum, PerkinElmer, USA; excitation, 745 nm; emission, 800 nm).
7
In Vivo Fluorescence Imaging and Photothermal Therapy
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Cy5.5-labeled FePS@PP or FePS@PPF was intravenously injected with 10 mg/kg FePS NSs for tumor-bearing mouse. The in vivo fluorescence images were acquired by Caliper IVIS Spectrum (PerkinElmer, USA) at designed time point (3, 6, 12, 24, 48 h). The ex vivo fluorescence of heart, liver, spleen, lung, kidney and tumor was detected at 24 h post-administration. All images were analyzed and calculated by Living Image software.
Mice were anaesthetized after 24 h-injection of PBS, FePS@PP or FePS@PPF, and the tumor sites were irradiated by 1064 nm laser for 5 min, 1.0 W/cm2. The temperature of tumor site was monitored using an infrared thermal imager.
Mice were anaesthetized after 24 h-injection of PBS, FePS@PP or FePS@PPF, and the tumor sites were irradiated by 1064 nm laser for 5 min, 1.0 W/cm2. The temperature of tumor site was monitored using an infrared thermal imager.
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