Log In Sign up
The largest database of trusted experimental protocols

Luna 5u

Manufactured by Phenomenex
Visit Supplier
Sourced in United States

The Luna 5u is a high-performance liquid chromatography (HPLC) column designed for analytical separations. It features a 5 micron particle size and is packed with a proprietary stationary phase material. The Luna 5u column provides efficient and reproducible separations of a wide range of analytes.

Automatically generated - may contain errors

Lab products found in correlation

Lc 8a, by Shimadzu (1 mentions) Chromquest software, by Thermo Fisher Scientific (1 mentions) Autosampler, by Agilent Technologies (1 mentions) Diode array detector, by Agilent Technologies (1 mentions) Agilent 1100 binary pump, by Agilent Technologies (1 mentions)

3 protocols using luna 5u

1

Analytical and Preparative RP-HPLC Peptide Purification

Check if the same lab product or an alternative is used in the 5 most similar protocols
Analytical RP-HPLC was carried out using a C18 column (Phenomenex, Luna 5u, 4.6mm X 250mm). The peptide samples were eluted from the column by employing a linear gradient of solvent B (5–90% B in 130 min; solvent B, 80% acetonitrile in water containing 0.1% aqueous TFA; solvent A, 0.1% TFA; flow rate: 1 ml/min). Preparative RP-HPLC (LC-8A Shimadzu Preparative Liquid Chromatograph) was used for large-scale purification of peptides using a preparative column (Phenomenex preparatory C18 column, Luna 10 μ, 30 mm X 250 mm). The chromatography was carried out using the same solvent gradient system as above but at a flow rate of 30 ml/min.
Singh S., Gupta K., Shukla S., Sampathkumar S.G, & Roy R.P. (2019). Sortase-click strategy for defined protein conjugation on a heptavalent cyclodextrin scaffold. PLoS ONE, 14(5), e0217369.
+ Open protocol
+ Expand
2

Purification and Characterization of Antioxidant Peptides

Check if the same lab product or an alternative is used in the 5 most similar protocols
The protein fraction obtained by gel filtration chromatography with the highest NO radical scavenging activity (F2) was further fractionated by RP-HPLC using a C18 column (250 mm×4.6 mm, Luna 5U; Phenomenex, Torrance, CA, USA). The peptides were eluted at room temperature using a gradient (0–100% mobile phase B for 20 min) of mobile phase A (0.1%, V/V, trifluoroacetic acid; TFA) and B (70%, V/V, ACN in 0.05%, V/V, TFA) at a flow rate of 0.7 mL/min. Chromatographic analyses were performed using the ChromQuest software (Thermo Fisher Scientific Inc., Waltham, MA, USA) (22 (link)). Peptides were detected by measuring the absorbance at A280 nm, the principal subfractions (F2-1 to F2-5) were isolated and collected, and the NO radical scavenging activity of three aliquots was determined while the rest was lyophilized.
Inkanuwat A., Sukaboon R., Reamtong O., Asawanonda P., Pattaratanakun A., Saisavoey T., Sangtanoo P, & Karnchanatat A. (2019). Nitric Oxide Synthesis Inhibition and Anti-Inflammatory Effect
of Polypeptide Isolated from Chicken Feather Meal
in Lipopolysaccharide-Stimulated RAW 264.7 Macrophages. Food Technology and Biotechnology, 57(2), 200-212.
+ Open protocol
+ Expand
3

Optimized LC-MS Protocol for Compound Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols
Liquid chromatography–mass spectrometry (LC-MS) was measured on a Bruker Daltonik GmbH, Germany, equipped with diode array detector (Agilent Technologies), Agilent 1100 binary pump and Autosampler (Agilent). The electrospray ionization positive mass spectra were acquired using a micrOTOF series mass spectrometer. Conditions, optimized using flow injection of standard and sample solutions, were as follows: electrospray ionization capillary voltage, 4.5 kV; end plate offset voltage, −500 V; nebulizer pressure, 2 bar; dry gas flow, 6 L/min; dry gas temperature 180 °C; source detector voltage 1,600 V; and TOF detector voltage 2,190 V. The electrospray ionization gas was nitrogen. All TOF measurements were performed at high resolution and the TOF analyzer was scanned at m/z 50–1,000 with a 1-s integration time. A flow rate of 0.4 mL/min was used for the analytical column C-8 (2), Phenomenex, Luna 5u, 150×4.6 mm. HPLC grade water and acetonitrile both containing 0.1 % formic acid were used as the mobile phase with a 15-min elution time.
Kasanah N., Farr L.L., Gholipour A., Wedge D.E, & Hamann M.T. (2014). Metabolism and Resistance of Fusarium spp. to the Manzamine Alkaloids via a Putative Retro Pictet-Spengler Reaction and Utility of the Rational Design of Antimalarial and Antifungal Agents. Marine biotechnology (New York, N.Y.), 16(4), 412-422.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!