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Signalsilence

Manufactured by Cell Signaling Technology
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SignalSilence is a laboratory reagent that enables the specific knockdown of target gene expression. It contains small interfering RNA (siRNA) designed to silence the expression of a particular gene of interest.

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Lab products found in correlation

Lipofectamine rnaimax, by Thermo Fisher Scientific (3 mentions) Rhtrail, by R&D Systems (1 mentions) Cd133 apc, by Miltenyi Biotec (1 mentions) Jnk in 8, by Selleck Chemicals (1 mentions) Phospho c jun, by Cell Signaling Technology (1 mentions) Fugene hd transfection reagent, by Promega (1 mentions) Gapdh antibody, by Santa Cruz Biotechnology (1 mentions) Dual luciferase reporter assay system, by Promega (1 mentions) Sv40 promoter, by Promega (1 mentions) Lipofectamine 3000, by Thermo Fisher Scientific (1 mentions) Control sirna, by Cell Signaling Technology (1 mentions) Silencer atf4 sirna, by Thermo Fisher Scientific (1 mentions) Opti mem, by Thermo Fisher Scientific (1 mentions) Lipofectamine rnaimax transfection reagent, by Thermo Fisher Scientific (1 mentions) Signalsilence control sirna, by Cell Signaling Technology (1 mentions) Fetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions) Silencer select, by Thermo Fisher Scientific (1 mentions)

Spelling variants of this product

SignalSilence® Control siRNA (28 citations) SignalSilence® p44/42 MAPK (Erk1/2) siRNA (5 citations) SignalSilence Control siRNA (unconjugated) (3 citations) SignalSilence® STAT3 siRNA (3 citations) SignalSilence control siRNA fluorescein conjugate (3 citations) SignalSilence® NF-κB p65 siRNA (3 citations) SignalSilence® p38α MAPK siRNA II (2 citations) SignalSilence® PERK siRNA I (2 citations) SignalSilence p38 MAPK siRNA (2 citations) SignalSilence JNK siRNA (2 citations)

6 protocols using signalsilence

1

Assaying JNK Inhibitor Efficacy in TRAIL-Induced Apoptosis

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Jun N-terminal kinase inhibitor II SP600125 (JNKi) was obtained from Calbiochem, JNK-IN-8 from Selleckchem, recombinant human TRAIL (rhTRAIL) from R&D Systems, and Gemcitabine from Elly Lilly. Products were reconstituted as recommended by the manufacturer.
The following antibodies were used: phospho-c-Jun (Cell Signaling Technologies), CD133-APC (Miltenyi Biotech), SSEA1-FITC (Santa Cruz Biotechnology, Inc.), DR4 antibody-PE Mouse IgG1B, DR5 antibody-FITC Mouse IgG2B and DcR1 antibody-APC Mouse IgG1 (R&D Systems).
Small interfering RNA for SAPK/JNK (#6232) and control (#6568) was obtained from SignalSilence ® (Cell Signaling Technologies).
Recio-Boiles A., Ilmer M., Rhea P.R., Kettlun C., Heinemann M.L., Ruetering J., Vykoukal J, & Alt E. (2016). JNK pathway inhibition selectively primes pancreatic cancer stem cells to TRAIL-induced apoptosis without affecting the physiology of normal tissue resident stem cells. Oncotarget, 7(9), 9890-9906.
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2

Allele-Specific Luciferase Assay of rs12291063

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250bp sequences containing either the major or the minor allele of rs12291063 were cloned into pGL3 Luciferase Reporter Vector driven by an SV40 promoter (Promega, Madison, WI) at the Hindlll site between the promoter and the firefly Luc gene. HEK-293 cells were cultured and transiently transfected with the constructs using FuGENE HD Transfection Reagent (Promega). For luciferase activity assay with hnRNPD depletion, HEK293 cells were transiently co-transfected with HNRNP siRNA or Control siRNA (SignalSilence, Cell Signaling Technology, MA). Dual Luciferase Reporter Assay System (Promega) was used to quantify firefly luciferase activity. Efficiency of siRNA knockdown for hnRNPD was verified by Western blot using pan-hnRNP antibody and GAPDH antibody (Santa Cruz Technology).
Mou Z., Hyde T.M., Lipska B.K., Martinowich K., Wei P., Ong C.J., Hunter L.A., Palaguachi G.I., Morgun E., Teng R., Lai C., Condarco T.A., Demidowich A.P., Krause A.J., Marshall L.J., Haack K., Voruganti V.S., Cole S.A., Butte N.F., Comuzzie A.G., Nalls M.A., Zonderman A.B., Singleton A.B., Evans M.K., Martin B., Maudsley S., Tsao J.W., Kleinman J.E., Yanovski J.A, & Han J.C. (2015). Human Obesity Associated with an Intronic SNP in the Brain-Derived Neurotrophic Factor Locus. Cell reports, 13(6), 1073-1080.
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3

Silencing of c-Jun and MAPKs in A549 Cells

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c-Jun and MAPKs were silenced in A549 cells using 100 nM of siRNA for c-Jun and JNK and, 25 nM for p38 and ERK (SignalSilence®, Cell Signaling Technology) that were transfected with Lipofectamine™ RNAiMAX (Invitrogen) following the manufacturer's recommendations. Controls were carried out using SignalSilence® Control siRNA (SCR). Subsequent to this, cells were exposed or not to the staphylococcal α-toxin followed by Western blot analyses.
Moyano A.J., Racca A.C., Soria G., Saka H.A., Andreoli V., Smania A.M., Sola C, & Bocco J.L. (2018). c-Jun Proto-Oncoprotein Plays a Protective Role in Lung Epithelial Cells Exposed to Staphylococcal α-Toxin. Frontiers in Cellular and Infection Microbiology, 8, 170.
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4

ATG7 Knockdown Using siRNA

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siRNA specific to ATG7 (#6604S) and a control siRNA (#6568S) were obtained from SignalSilence (Cell Signaling Technology, Inc.). We seeded cells on 6-well plate (2×105 cells/well) 16 hrs before siRNA transfection. Transfection was performed using LipofectAMINE 3000 (Invitrogen) according to the manufacturer's instructions. A total of 100 nmol/L of siRNA duplex was used for each transfection.
Yan C., Luo L., Goto S., Urata Y., Guo C.Y., Doi H., Kitazato K, & Li T.S. (2016). Enhanced autophagy in colorectal cancer stem cells does not contribute to radio-resistance. Oncotarget, 7(29), 45112-45121.
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5

Silencing of MAPK and CHOP in HepG2 cells

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HepG2 cells were reverse transfected with SignalSilence® p44/42 MAPK (ERK1/2) siRNA, SignalSilence® Control siRNA (Cell Signaling Technology, Danvers, MA), Silencer® ATF4 siRNA, Silencer® CHOP siRNA, or Silencer® Negative Control #1 siRNA (Life Technologies) using Lipofectamine® RNAiMAX Transfection Reagent (Life Technologies). In brief, HepG2 cells were dissociated, and seeded to each 10 cm dish at a density of 3 × 106 cells/dish in 10 ml medium composed of 8 ml of antibiotic-free growth medium, 2 ml of Opti-MEM (Life Technologies), 20 µl of Lipofectamine RNAiMAX, and 200 pmol of desired siRNA. After 24 h of transfection, cells were changed to normal growth medium and plated into 96-well plates and/or 6 cm plates, as described before. Drug treatment started 48 h post siRNA transfection. The silencing efficiency of the siRNAs was determined by Western blotting.
Ren Z., Chen S., Qing T., Xuan J., Couch L., Yu D., Ning B., Shi L, & Guo L. (2017). Endoplasmic reticulum stress and MAPK signaling pathway activation underlie leflunomide-induced toxicity in HepG2 Cells. Toxicology, 392, 11-21.
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6

Autophagy Regulation in Breast Cancer Cells

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MCF7, T47D, BT-474, and MDA-MB-231 cells were maintained in improved minimal essential media (IMEM) with phenol red and supplemented with 5% fetal bovine serum (Life Technologies). MCF7/LCC1 (LCC1) and MCF7/LCC9 (LCC9) cells were grown in phenol red-free IMEM supplemented with 5% charcoal-stripped calf serum. All cells were maintained in a humidified atmosphere at 37°C and 95% air/5% CO2. ATG7 (SignalSilence; Cell Signaling Technology), BECN1 and STAT1 (three unique siRNAs for each target; OriGene), IRF1 (Silencer Select; consisting of 3 different siRNA for same target; Life Technologies), or control (Ctrl) siRNA were transfected in cells using Lipofectamine RNAiMAX (Life Technologies) according to the manufacturer's instructions. ERα and ATG7 cDNA were from Origene; ICI 182,780 (ICI; Faslodex; Fulvestrant) was from Tocris Bioscience; HCQ, 3-MA, and NAC were from Sigma-Aldrich and the JAK inhibitor 1 (C19H16FN3O) was obtained from EMD Millipore.
Schwartz-Roberts J.L., Cook K.L., Chen C., Shajahan-Haq A.N., Axelrod M., Wärri A., Riggins R.B., Jin L., Haddad B.R., Kallakury B.V., Baumann W.T, & Clarke R. (2015). Interferon Regulatory Factor-1 signaling regulates the switch between autophagy and apoptosis to determine breast cancer cell fate. Cancer research, 75(6), 1046-1055.
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