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4 protocols using salmonella enteritidis cvcc 3377

1

Bacterial Strain Acquisition for Research

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Strains of E. coli CVCC 1515, Salmonella enteritidis CVCC 3377 and Salmonella pullorum CVCC 503 were purchased from the China Veterinary Culture Collection Center (CVCC) (Beijing, China). Shigella dysenteriae CMCC 51252 and Shigella flexneri CMCC 51571 were purchased from the National Center for Medical Culture Collection (CMCC) (Beijing, China). E. coli CICC 21530 (O157:H7), Pseudomonas aeruginosa CICC 10419 and CICC 21630 strains were purchased from the China Center of Industrial Culture Collection (CICC) (Beijing, China). E. coli DH5α and BL21 (DE3) strains were purchased form TransGen Biotech Co., Ltd. (Beijing, China). The pMD™ 19-T Simple and pET-28a(+) vectors with His tags were obtained from TaKaRa Biotechology Co., Ltd. (Dalian China) and Novagen (America), respectively.
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2

Bacterial Strains and Cell Line Acquisition

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The bacterial strains S. aureus ATCC 43300, Staphylococcus epidermidis ATCC 12228, and Escherichia coli ATCC 25922 were purchased from American Type Culture Collection (ATCC). S. aureus CVCC 546, Streptococcus pneumoniae CVCC 2350, Streptococcus suis CVCC 3928, Salmonella pullorum CVCC 533, Salmonella enteritidis CVCC 3377, and E. coli K88 were purchased from the China Veterinary Culture Collection Center (CVCC). RAW 264.7 murine macrophages were obtained from Peking Union Medical College.
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3

Microbial Strain Collection Protocol

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E. coli O78 (CVCC CAU0768) was donated by Professor Aike Li from the Academy of National Food and Strategic Reserves Administration. Shigellasonnei CVCC 3926 was donated by Professor Bo Shi from the Feed Research Institute of Chinese Academy of Agricultural Sciences. Salmonella enteritidis CVCC 3377 were bought from the China Veterinary Culture Collection Center (CVCC) (Beijing, China). Sh. flexneri CMCC (B) 51571 was purchased from the National Center for Medical Culture Collection (CMCC) (Beijing, China). Pseudomonas aeruginosa CICC 21630, P. aeruginosa CICC 10419 and E. coli O157 (CICC 21530) were purchased from the China Center of Industrial Culture Collection (CICC) (Beijing, China). E. coli competent strains of BL21 (DE3) and DH5α were purchased from TransGen Biotech Co., Ltd. (Beijing, China). The vector of pET-28a (+) was purchased from Novagen (Madison, WI, USA) and pMDTM 19-T Simple was purchased from TaKaRa Biotechnology (Dalian, China).
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4

Antimicrobial Activity of Peptides ID13 and DLP4

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The bacterial strains Staphylococcus epidermidis ATCC 12228 and Escherichia coli ATCC 25922 were purchased from American Type Culture Collection (ATCC). S. aureus CVCC 546, Streptococcus pneumoniae CVCC 2350, Streptococcus suis CVCC 3928, Salmonella pullorum CVCC 533, and Salmonella Enteritidis CVCC 3377 were purchased from the China Veterinary Culture Collection Center (CVCC). Mouse macrophages RAW 264.7 and endometrial epithelial cells (MEECs) were obtained from Peking Union Medical College and iCell Bioscience Inc. (Shanghai, China), respectively. LTA and 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide (MTT) were purchased from Sigma-Aldrich (China). Antibiotics used in the study were purchased from Meilun Biotech Co., Ltd (Dalian, China). Peptides ID13 and DLP4 were expressed and purified in our lab as previously described (Li et al. 2020 ), with the purity of 91.2% and 92%, respectively. Other reagents were of analytical grade.
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