The anti-HAV antibody titers of all IVIG preparations were determined by electro-chemiluminescence immunoassay using an Elecsys Anti-HAV II kit and a Cobas 8000 e602 analyzer (Roche Diagnostics, Mannheim, Germany). Anti-hepatitis B surface (anti-HBs) antibody titers were measured by chemiluminescent microparticle immunoassay using an ARCHITECT Anti-HBs Reagent Kit and an ARCHITECT i400 immunoassay analyzer (Abbott Laboratories, Lake Bluff, IL, USA). These experiments were performed by Seegene Medical Foundation (Seoul, Korea). The lower limits of detection for anti-HAV and anti-HBs antibodies were 3 mIU/mL and 10 mIU/mL, respectively, in this assay system.
Architect anti hbs reagent kit
Manufactured by Abbott
Sourced in United States
The ARCHITECT Anti-HBs Reagent Kit is a laboratory diagnostic product manufactured by Abbott. It is designed to quantitatively determine the presence and level of antibodies to the hepatitis B surface antigen (anti-HBs) in human serum or plasma samples.
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Lab products found in correlation
4 protocols using architect anti hbs reagent kit
1
Determination of Antibody Titers in IVIG
2
Infant HBV Vaccine Response Evaluation
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Venous blood samples were collected from infants at the age of 7 months (1 month after the last dose of the vaccine). The detection of HBsAg in infants was carried out through the chemiluminescent microparticle immunoassay (CMIA) with an Abbott ARCHITECT HBsAg Reagent Kit (Abbott Laboratories, North Chicago, Illinois, USA). An ARCHITECT anti-HBs Reagent Kit (Abbott Laboratories) from CMIA was used to determine the anti-HBs levels, with a range of 0–15000 mIU/mL after 15 dilutions. Infants with serum anti-HBs <100 mIU/mL and anti-HBs ≥100 mIU/mL at 7 months of age were classified as low responders (LRs) and high responders (HRs), respectively. Maternal HBV DNA was assessed by the Roche TaqMan HBV test (Roche Diagnostics, Grenzach, Germany).
3
Monitoring Hepatitis B Serum Markers
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The following were measured from blood serum samples collected before and at the end of DAA therapy: levels of HBsAg and anti-HBs, hepatitis B core antibodies (anti-HBc), and HBV-DNA. Methods used for measurements were as follows: chemiluminescent immunoassay (CLIA) for HBs antigens (ARCHITECT HBsAg Reagent Kit; Abbott, Chicago, IL); CLIA for HBs antibodies (ARCHITECT Anti-HBs Reagent Kit; Abbott); CLIA for HBc antibodies (ARCHITECT Anti-HBc II Reagent Kit; Abbott), and the TaqMan probe for HBV-DNA (COBAS Ampriprep/COBAS TaqMan HBV Test, v2.0; Roche, Basel, Switzerland).
4
Quantifying Anti-HBs Reactivity with HBV Variants
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To determine the reactivity of anti-HBs with wildtype SHBs-containing subviral particles of HBV subgenotype D3, the quantitative anti-HBs assay (Abbott ARCHITECT Anti-HBs Reagent Kit, 7C1839) was used. Variant SHBs-containing cell culture supernatants were diluted with PBS to adjust variant HBsAg to equal amounts as calculated via immunoblot quantification (see above) which were equivalent to 10 IU/mL wildtype HBsAg. After the addition of 50 IU/L anti-HBs (final concentration) present in various types of human sera, samples were incubated for 1 h at 37 °C and then measured with the anti-HBs assay. The human sera came from three groups with six subjects each: (i) HBV vaccinees (anti-HBs positive, anti-HBc negative), (ii) recovered from HBV infection (anti-HBs positive, anti-HBc positive), and (iii) HBV naïve (anti-HBs negative, anti-HBc negative).
Patient sera were obtained at the University Clinic Giessen in accordance with the local ethics committee of the Department of Medicine of the Justus Liebig University Giessen (AZ 257/18).
Patient sera were obtained at the University Clinic Giessen in accordance with the local ethics committee of the Department of Medicine of the Justus Liebig University Giessen (AZ 257/18).
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