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Sulforaphane and Didox Protocol

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Sulforaphane (SF, purity >98 %) was purchased from Sigma-Aldrich (St. Louis, MO, USA). Didox (purity >98 %) was purchased from MedChem Express (Monmouth Junction, ON, USA). Solvents used for extraction were of analytical grade and obtained from Tianjin Fuyu Chemical Company (Tianjin, China).
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2

Biochemicals and Reagents for Cell Experiments

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Sulforaphane (SF), menadione, digitonin, As(III), and flavin adenine dinucleotide were purchased from Sigma-Aldrich (MO, USA). Didox was purchased from MedChemExpress (Monmouth Junction, NJ, USA). Cycloheximide (CHX), 4′,6-diamidino-2-phenylindole (DAPI), 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazoliumbromide (MTT), bromophenol blue, glycerol, and bovine serum albumin (BSA) were purchased from Genview (TX, USA). β-Mercaptoethanol was obtained from Dingguo (Beijing, China). Eagle's minimal essential medium (MEM) and RPMI 1640 were acquired from Gibco (CA, USA). Fetal bovine serum (FBS) was purchased from Gemini Bio Products (CA, USA). Glucose-6-phosphate and L-glutamine were obtained from Solarbio (Beijing, China). Glucose-6-phosphate dehydrogenase and nicotinamide adenine dinucleotide phosphate (NADP) were obtained from Regal (Shanghai, China). Solvents used for extraction and isolation were of analytical grade and obtained from Tianjin Fuyu Fine Chemical Company (Tianjin, China). Naphthylethylenediamine and sulfanilamide were obtained from Sinopharm Chemical Reagent Co. Ltd. (China).
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