9 thc d3

Six of the seven cannabinoid analytes [∆⁹-THC, cannabinol (CBN), cannabigerol (CBG), cannabigerolic acid (CBGA), ∆⁹-tetrahydrocannabinolic acid A (∆⁹-THCA), and ∆⁹-tetrahydrocannabivarin (∆⁹-THCV)] and the internal standard (IS; ∆⁹-THC-D₃) were obtained as certified reference materials (CRMs) manufactured by Cerilliant (Round Rock, TX). Cannabichromene (CBC) was obtained as a CRM from Cayman Chemical (Ann Arbor, MI). When not in use, concentrated stock solutions of these agents and working solutions made therefrom were stored at –20 °C.
Acetonitrile, formic acid, methanol, and n-hexane were purchased from Thermo Fisher Scientific and were of LC/MS grade. Ethyl acetate (Acros Organics) was purchased from Thermo Fisher Scientific and was of spectroscopy grade (> 99.5%). High purity water (18.2 MΩ) required for preparing the mobile phase and for sample extraction was produced using an EMD Millipore Simplicity water purification system. When not in use, these agents were stored at room temperature (20–25 °C). Nitrogen (N₂), supplied as a cryogenic liquid in a 230L dewar at a purity of 99.998%, or as compressed nitrogen gas at a purity of 99.999% in T-type cylinders, was obtained from Praxair (Danbury, CT).

Eight of the 10 cannabinoid analytes [∆⁹-THC, CBN, cannabigerol (CBG), cannabigerolic acid (CBGA), ∆⁹-tetrahydrocannabinolic acid A (∆⁹-THCA), ∆⁹-tetrahydrocannabivarin (∆⁹-THCV), 11-OH-∆⁹-THC, and 11-nor-9-carboxy-∆⁹-THC] and the internal standard (IS; ∆⁹-THC-D₃) were obtained as certified reference materials (CRMs) manufactured by Cerilliant (Round Rock, TX). The 8β,11-diOH-Δ⁹-THC metabolite was obtained from ElSohly Laboratories, Inc. (Oxford, MS). Cannabichromene (CBC) was obtained as a CRM from Cayman Chemical (Ann Arbor, MI). When not in use, concentrated stock solutions of these agents and working solutions made therefrom were stored at – 20 °C.
Acetonitrile, formic acid, methanol, and n-hexane were purchased from Thermo Fisher Scientific and were of LC/MS grade. Ethyl acetate (Acros Organics) was purchased from Thermo Fisher Scientific and was of spectroscopy grade (> 99.5%). High purity water (18.2 MΩ) required for preparing the mobile phase and for sample extraction was produced using an EMD Millipore Simplicity water purification system. When not in use, these agents were stored at room temperature (20–25 °C). Nitrogen (N₂), supplied as a cryogenic liquid in a 230L dewar at a purity of 99.998%, or as compressed nitrogen gas at a purity of 99.999% in T-type cylinders, was obtained from Praxair (Danbury, CT).