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Ptc 225 dna engine

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The PTC-225 DNA Engine is a thermal cycler used for DNA amplification and analysis. It is capable of precise temperature control and cycling to facilitate the polymerase chain reaction (PCR) process.

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Lab products found in correlation

Taq dna polymerase, by Qiagen (2 mentions) Superscript 3 first strand synthesis protocol, by Thermo Fisher Scientific (1 mentions) Qiaquick gel extraction kit, by Qiagen (1 mentions)

Spelling variants of this product

DNA Engine (36 citations) PTC-225 (12 citations) PTC-225 DNA Engine Tetrad Peltier (2 citations)

2 protocols using ptc 225 dna engine

1

WT1 Gene Expression Profiling

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cDNA was made according to the SuperScript III First-Strand Synthesis protocol (Life Technologies) using up to 5 μg total RNA, 2.5 μM oligo(dT)20, and 500 μM dNTP mix. WT1-cDNA was amplified using a mixture of 2 μl cDNA, 300 nM Ws-Ex6f (see S3 Table for primers), 300 nM Ws-Ex10r, 200 μM dNTP mix, and 1 U Taq DNA polymerase (Qiagen) on PTC-225 DNA Engine (MJ Research) for 35 cycles with denaturation and annealing temperatures of 93°C and 58°C, respectively. The amplicons were then gel-purified as described above.
Niavarani A., Currie E., Reyal Y., Anjos-Afonso F., Horswell S., Griessinger E., Luis Sardina J, & Bonnet D. (2015). APOBEC3A Is Implicated in a Novel Class of G-to-A mRNA Editing in WT1 Transcripts. PLoS ONE, 10(3), e0120089.
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2

WT1 Exon 7 and 9 Amplification

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WT1 exon 7 was amplified using 20–50 ng of gDNA, 200 nM Ws-X7f5 (see S3 Table for primers), 200 nM Ws-X7r5, 200 μM dNTP mix, and 1 U Taq DNA Polymerase (Qiagen) on a PTC-225 DNA Engine (MJ Research) for 35 cycles with annealing at 61°C. WT1 exon 9 was also amplified according to the same protocol, but using Ws-X9f3 and Ws-X9r2 primers. The amplicons were then gel-extracted using Qiaquick Gel Extraction Kit (Qiagen), making it ready for cloning or direct Sanger sequencing.
Niavarani A., Currie E., Reyal Y., Anjos-Afonso F., Horswell S., Griessinger E., Luis Sardina J, & Bonnet D. (2015). APOBEC3A Is Implicated in a Novel Class of G-to-A mRNA Editing in WT1 Transcripts. PLoS ONE, 10(3), e0120089.
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