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The CD122 is a laboratory instrument designed for the detection and analysis of specific cell surface markers. It utilizes flow cytometry technology to identify and quantify the expression of the CD122 protein on cells. The core function of the CD122 is to provide researchers with accurate and reliable data on the presence and levels of the CD122 marker in cell samples.

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Lab products found in correlation

Antibodies against mouse, by Thermo Fisher Scientific (2 mentions) Anti human cd101, by Abcam (2 mentions) Facscanto 2, by BD (2 mentions) Il 10, by BD (1 mentions) Interleukin 4 (il 4), by BD (1 mentions) Il 22, by BD (1 mentions) Cd154, by BD (1 mentions) Cd62l, by BD (1 mentions) Ifn γ, by BD (1 mentions) Klrg1, by BioLegend (1 mentions) Il 17, by BD (1 mentions) Cxcl8, by BD (1 mentions) Reparixin, by MedChemExpress (1 mentions) Cxcr2, by BD (1 mentions) Cd62l, by Thermo Fisher Scientific (1 mentions) Mhcii, by Thermo Fisher Scientific (1 mentions) H 2kb, by Thermo Fisher Scientific (1 mentions) Anti mouse tcrβ, by Thermo Fisher Scientific (1 mentions) H 2kd, by Thermo Fisher Scientific (1 mentions) Cd172a, by Thermo Fisher Scientific (1 mentions)

Spelling variants of this product

CD122 (TM-b1; (4 citations) PE-Cy7-CD122 (2 citations) Anti‐CD122‐APC (2 citations)

5 protocols using cd122

1

Multiparametric Flow Cytometry Analysis

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Antibodies against mouse TCRβ, CD4, CD101, CD25, Cd122, CD45.1, CD45.2, FoxP3, CD11b and CD11c were purchased from eBioscience. Antibodies against human CD3, CD4, CD25 and CD11b were purchased from eBioscience; anti-human CD101 was obtained from Abcam. Intracellular staining for Foxp3 and intracellular cytokines was performed with a staining kit (eBioscience) following the manufacturer’s instructions. Cells were analyzed on a BD FACS Canto II (BD Biosciences, San Diego, CA) with FlowJo software (Tree Star).
Schey R., Dornhoff H., Baier J.L., Purtak M., Opoka R., Koller A.K., Atreya R., Rau T.T., Daniel C., Amann K., Bogdan C, & Mattner J. (2016). CD101 inhibits the expansion of colitogenic T cells. Mucosal immunology, 9(5), 1205-1217.
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2

Multiparameter Analysis of T Cell Responses

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Recombinant-human cytokines and chemokines were from R&D systems. Antibodies for flow cytometry were CCR7, CD3, CD4, CD8, CD25, CD31, CD45RA, CD45RO, CD62L, CD69, CD154, CXCR2, CXCR3, CXCL8, IFN-γ, IL-4, IL-10, IL-17, and IL-22 (BD), CD57, CD122, Foxp3 (eBiosciences/ThermoFischer), KLRG-1 (BioLegend). Antibodies for T cell stimulation were CD3, CD28 (BD or Affymetrix). Small-molecule CXCR1/2 chemical inhibitor Reparixin (RPX) was purchased from MedChem Express.
Crespo J., Wu K., Li W., Kryczek I., Maj T., Vatan L., Wei S., Opipari A.W, & Zou W. (2018). Human naïve T cells express functional CXCL8 and promote tumorigenesis. Journal of immunology (Baltimore, Md. : 1950), 201(2), 814-820.
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3

Multiparametric Flow Cytometry Analysis

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Antibodies against mouse TCRβ, CD4, CD101, CD25, Cd122, CD45.1, CD45.2, FoxP3, CD11b and CD11c were purchased from eBioscience. Antibodies against human CD3, CD4, CD25 and CD11b were purchased from eBioscience; anti-human CD101 was obtained from Abcam. Intracellular staining for Foxp3 and intracellular cytokines was performed with a staining kit (eBioscience) following the manufacturer’s instructions. Cells were analyzed on a BD FACS Canto II (BD Biosciences, San Diego, CA) with FlowJo software (Tree Star).
Schey R., Dornhoff H., Baier J.L., Purtak M., Opoka R., Koller A.K., Atreya R., Rau T.T., Daniel C., Amann K., Bogdan C, & Mattner J. (2016). CD101 inhibits the expansion of colitogenic T cells. Mucosal immunology, 9(5), 1205-1217.
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4

Isolation and Characterization of Mouse Immune Cells

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Antibodies including anti-mouse TCRβ, CD4, CD8, CD44, CD62L, H-2Kb, H-2Kd, CD122, CD69, CD137, MHC-II, CD86, CD70, CD24, CD172a, and B220 were purchased from eBioscience. CD90.2 microbeads and negative mouse CD8+ T cells isolation kits were purchased from Miltenyi Biotec and Stem Cell Company respectively. The mouse CD11c+ cell isolation kits for CD11c+ negative selection were purchased from Stem Cell Company.
Mohammadpour H., O’Neil R., Qiu J., McCarthy P.L., Repasky E.A, & Cao X. (2018). Blockade of host β2-adrenergic receptor enhances graft-versus-tumor effect through modulating antigen presenting cells. Journal of immunology (Baltimore, Md. : 1950), 200(7), 2479-2488.
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5

Multiparametric Flow Cytometry of Splenocytes

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Fixed and permeabilized splenocytes were stained with fluorophore-conjugated antibodies purchased from either BD Biosciences (San Jose, CA) or eBioscience (San Diego, CA): B220(RA3-6B2), Bcl-6(K112-91), CD4(RM4), CD8α (53–6.7), CD45RB(C363.16A), CD45RB(C363.16 A), CD49b(DX5), CD122(TM-B1), Fas(Jo2), IgM(II/41), Ki67(B56), NK1.1(PK136), PD-1(J43). Qdm (AMAPRTLLL) and Preproinsulin II (ALWMRFLPL) peptides were synthesized by GenScript (Piscataway, NJ) and shipped to the NIH Tetramer Core (Emory University, Atlanta, GA) for folding into mouse Qa-1b Tetramers labeled with APC. Samples were acquired on a BD LSRFortessa and analyzed by FlowJo (TreeStar, Ashland, OR).
Stocks B.T., Wilson C.S., Marshall A.F., Brewer L.A, & Moore D.J. (2017). Host Expression of the CD8 Treg/NK Cell Restriction Element Qa-1 is Dispensable for Transplant Tolerance. Scientific Reports, 7, 11181.
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