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2 protocols using cd197 ccr7 g043h7

1

Characterization of T Cell Subsets

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Mouse specific antibodies against CD3ε/γ (17A2; Biolegend), CD4 (RM4-5; eBioscience), CD25 (PC61.5; eBioscience), CD44 (IM7; BD Biosciences), CD62L (MEL-14; Life Technologies), CD126 (D7715A7; eBioscience), CD127 (25-1271-82; eBioscience),βTCR (H57-597), gp130 (125623; R&D Systems), IFNγ (XMG1.2; eBioscience), IL-4 (11B11; eBioscience), IL-17A (TC11-18H10.1; Biolegend), IL-21 (Recombinant mouse IL-21R Fc Chimera protein; R&D and IL-21 receptor antibody; Jackson Immuno Research) and PTPN2 (AF1930; R&D) were used. For detection of human antigens, we used antibodies specific to CD3 (UCHT1; BioLegend), CD4 (RPA-T4; eBioscience), CD45RA (HI100; BioLegend), CD45RO (UCHL1; BioLegend), CD62L (DREG-56; BD Biosciences), CD197 (CCR7; G043H7; BioLegend). Human and mouse cross-specific antibodies to pY STAT1 (pY701; 4a), pY-STAT3 (pY705; 4/P-STAT3), pS-STAT1 (pS727; K51-856) and pS STAT3 (pS727, 49/p-Stat3) were from BD Biosciences.
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2

Characterization of T Cell Subsets

Check if the same lab product or an alternative is used in the 5 most similar protocols
Mouse specific antibodies against CD3ε/γ (17A2; Biolegend), CD4 (RM4-5; eBioscience), CD25 (PC61.5; eBioscience), CD44 (IM7; BD Biosciences), CD62L (MEL-14; Life Technologies), CD126 (D7715A7; eBioscience), CD127 (25-1271-82; eBioscience),βTCR (H57-597), gp130 (125623; R&D Systems), IFNγ (XMG1.2; eBioscience), IL-4 (11B11; eBioscience), IL-17A (TC11-18H10.1; Biolegend), IL-21 (Recombinant mouse IL-21R Fc Chimera protein; R&D and IL-21 receptor antibody; Jackson Immuno Research) and PTPN2 (AF1930; R&D) were used. For detection of human antigens, we used antibodies specific to CD3 (UCHT1; BioLegend), CD4 (RPA-T4; eBioscience), CD45RA (HI100; BioLegend), CD45RO (UCHL1; BioLegend), CD62L (DREG-56; BD Biosciences), CD197 (CCR7; G043H7; BioLegend). Human and mouse cross-specific antibodies to pY STAT1 (pY701; 4a), pY-STAT3 (pY705; 4/P-STAT3), pS-STAT1 (pS727; K51-856) and pS STAT3 (pS727, 49/p-Stat3) were from BD Biosciences.
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