PCR reactions were prepared using a GeXP Start Kit (Beckman Coulter, Brea, CA, USA) consisting of cDNA samples taken from the RT reaction (9.3 μL each), 5x PCR buffer (2 μL), 25 mM MgCl2 (2 μL), PCR multiplex forward primer (1 μL), and Thermo-Start DNA polymerase (0.7 μL). Amplification conditions were 95°C for 10 min, followed by 34 cycles of 94°C for 30 sec, 55°C for 30 sec, 70°C for 1 min, and 4°C hold carried out in an XP Thermal Cycler (BIOER Technology, Hangzhou, China) [18 (link)].
Gexp start kit
Manufactured by Beckman Coulter
Sourced in United States
The GeXP Start Kit is a laboratory equipment product used for automated nucleic acid sample preparation. It is designed to perform efficient and reliable extraction and purification of nucleic acids from a variety of sample types. The kit includes all necessary reagents and consumables required for the sample preparation process.
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Lab products found in correlation
2 protocols using gexp start kit
1
Real-time Multiplex PCR Amplification
2
Multiplex PCR Amplification Protocol
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PCR reactions were carried out using GeXP Start Kit (Beckman Coulter, Miami, FL, USA) consisting of cDNA sample taken from the RT reaction (9.3 μL each), 5X PCR buffer, 25 mM magnesium chloride, PCR multiplex forward primer and Thermo-Start DNA polymerase. Amplification was done in an XP Thermal Cycler (BIOER Technology, Hangzhou, China) using 95°C for 10 min, followed by 34 cycles of 94°C for 30 sec, 55°C for 30 sec, 70°C for 1 min and 4°C hold.
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