Phical

Manufactured by Immundiagnostik

Sourced in Germany

The PhiCal is a laboratory equipment designed for the detection and quantification of calprotectin, a biomarker associated with various inflammatory conditions. The product utilizes a immunochemical assay method to provide accurate and reliable measurements of calprotectin levels in biological samples. The core function of the PhiCal is to assist healthcare professionals in the diagnosis and monitoring of inflammatory disorders.

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Lab products found in correlation

Multiskan fc, by Thermo Fisher Scientific (2 mentions) Elisa kit, by Immundiagnostik (1 mentions)

5 protocols using phical

Quantitative Fecal Calprotectin Assay

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Collected fecal samples used for FC measurements were stored at −20 °C until shipment to the laboratory (SRL Inc., Tokyo, Japan), where the FC level was determined with the examiner blinded to the endoscopic findings using a quantitative enzyme-linked immunosorbent assay (PhiCal®, Immundiagnostik, Germany), with a standard method. The detection limits of this ELISA kit for FC range from 5.3 to 2100 μg/g.

Kawashima K., Ishihara S., Yuki T., Fukuba N., Oshima N., Kazumori H., Sonoyama H., Yamashita N., Tada Y., Kusunoki R., Oka A., Mishima Y., Moriyama I, & Kinoshita Y. (2016). Fecal calprotectin level correlated with both endoscopic severity and disease extent in ulcerative colitis. BMC Gastroenterology, 16, 47.

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Fecal Calprotectin ELISA Quantification

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Fecal calprotectin concentrations were measured with a quantitative PhiCal enzyme-linked immunosorbent assay (ELISA) kit (Immundiagnostik AG, catalog no. K6927) according to the manufacturer’s instructions. Fecal specimens were diluted 1:2,500. ELISA plates were read by the use of a Thermo Scientific microplate reader (Multiskan FC; optical density at 450 nm against 620 nm). Samples containing ≥100 μg of calprotectin per 1 g of feces were considered calprotectin positive (51 (link)).

Zhou Y., Xu Z.Z., He Y., Yang Y., Liu L., Lin Q., Nie Y., Li M., Zhi F., Liu S., Amir A., González A., Tripathi A., Chen M., Wu G.D., Knight R., Zhou H, & Chen Y. (2018). Gut Microbiota Offers Universal Biomarkers across Ethnicity in Inflammatory Bowel Disease Diagnosis and Infliximab Response Prediction. mSystems, 3(1), e00188-17.

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Fecal Calprotectin Measurement Protocol

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Fecal samples were collected twice at weeks 0 and 8. In patients withdrawn from the study due to exacerbation, fecal samples were collected on the day of study discontinuation. Fecal samples were stored in a freezer at −20°C until measurements. The calprotectin concentration was determined using a quantitative enzyme-linked immunosorbent assay (PhiCal, Immundiagnostik, Germany).

Kawashima K., Ishihara S., Yuki T., Onishi K., Kushiyama Y., Fujishiro H., Miyaoka Y., Yuki M., Komazawa Y., Tanimura T., Sonoyama H., Tada Y., Kusunoki R., Oka A., Fukuba N., Oshima N., Moriyama I, & Kinoshita Y. (2014). Therapeutic Efficacy of pH-Dependent Release Formulation of Mesalazine on Active Ulcerative Colitis Resistant to Time-Dependent Release Formulation: Analysis of Fecal Calprotectin Concentration. BioMed Research International, 2014, 342751.

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Fecal Calprotectin and Inflammatory Markers in Colonoscopy

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Fecal samples were collected within the 3-day period prior to undergoing a colonoscopy and stored at −20°C until transfer to an external laboratory (SRL Inc., Tokyo, Japan) for analysis. FC level was determined using an enzyme-linked immunosorbent assay method (PhiCal^®, Immundiagnostik AG, Germany). Blood samples were obtained on the day of the endoscopy prior to the procedure, with WBC, hemoglobin (Hb) level, platelet count (PLT), and ESR level, as well as serum high sensitive CRP and serum albumin (Alb) levels determined at the clinical laboratory of each hospital.

Sonoyama H., Kawashima K., Ishihara S., Kotani S., Fukuba N., Oka A., Kusunoki R., Tada Y., Mishima Y., Oshima N., Moriyama I., Yuki T., Onishi K, & Kinoshita Y. (2019). Capabilities of fecal calprotectin and blood biomarkers as surrogate endoscopic markers according to ulcerative colitis disease type. Journal of Clinical Biochemistry and Nutrition, 64(3), 265-270.

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Biomarker Analysis of Blood Samples

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Blood samples were analyzed for hemoglobin, erythrocyte sedimentation rate (ESR), and C-reactive protein (CRP) using standard laboratory techniques. Serum calprotectin was analyzed at baseline by ELISA (PhiCal; Immundiagnostik AG, Bensheim, Germany) following the manufacturer’s instructions. According to the manufacturer, the normal range for serum calprotectin is 500–3000 ng/ml.

Klingberg E., Strid H., Ståhl A., Deminger A., Carlsten H., Öhman L, & Forsblad-d’Elia H. (2017). A longitudinal study of fecal calprotectin and the development of inflammatory bowel disease in ankylosing spondylitis. Arthritis Research & Therapy, 19, 21.

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