Anti cd21 apc

Manufactured by BD

Anti-CD21 APC is a fluorescently-labeled antibody that binds to the CD21 cell surface antigen. CD21 is a complement receptor expressed on B cells and a subset of T cells. The Anti-CD21 APC antibody can be used to detect and analyze these cell populations in flow cytometry applications.

Automatically generated - may contain errors

Lab products found in correlation

Spelling variants of this product

CD21-APC (9 citations) Anti-CD21 (7 citations)

5 protocols using anti cd21 apc

Multiparametric Analysis of Splenic Lymphocytes

Check if the same lab product or an alternative is used in the 5 most similar protocols

Splenic lymphocytes were stained for surface markers as follows. For extracellular markers, single cells were stained at 2 × 10⁶ cells per well in a 96-well V bottomed plate. T cells were stained with anti-CD3 Alexa 700 (BD, clone 500A2), anti-CD4 PerCP (BD, clone RM4-5), anti-CD44 FITC (BD, clone IM7), anti-CD62L V450 (BD, clone MEL-14), and anti-FOXP3 APC (BD, clone MF23). B cells were stained with anti-CD19 PEcy7 (eBiosciences, clone 6D5), anti-B220 FITC (eBiosciences, clone RA3-6B2), anti-CD21 APC (BD, clone 7G6), anti-CD23 PE (BD), and anti-CD80 V450 (BD, clone 16-10A1). Fifty microliters of the antibody master mix prepared in MACS buffer (1× PBS, 2 mM EDTA, and 0.5% BSA) was added per well in all staining procedures. Cells were acquired on an LSRII (Becton Dickinson) and analyzed by FlowJo (Tree Star, Ashland).

Nyangahu D.D., Lennard K.S., Brown B.P., Darby M.G., Wendoh J.M., Havyarimana E., Smith P., Butcher J., Stintzi A., Mulder N., Horsnell W, & Jaspan H.B. (2018). Disruption of maternal gut microbiota during gestation alters offspring microbiota and immunity. Microbiome, 6, 124.

+ Open protocol

+ Expand

Comprehensive B Cell Subset Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols

The following Abs were used: anti-CD19–ECD, anti-CD27–Pe-Cy5, anti-CD10–FITC, anti-IgD–PE (Beckman Coulter); anti-CD20–APC-H7, anti-CD21–APC (BD Biosciences); anti-CD21–PE, anti-CD38–Pe-Cy7, anti-BR3–FITC (eBioscience), and anti–transmembrane activator and calcium modulator and cyclophilin ligand interactor (TACI)–PE (R&D Systems).
Whole blood was washed and stained with cocktails comprising the above Abs, and the RBCs were lysed. At least 80,000 PBMCs were acquired on a nine-color CyAn ADP flow cytometer (Beckman Coulter). The data were analyzed using FlowJo software version 9.2 (TreeStar, FlowJo Africa scheme).
The following B cell subsets were identified based on indicated surface markers: immature/transitional, CD19⁺CD10⁺CD38⁺⁺CD27⁻; naive, CD19⁺CD27⁻CD21⁺; tissue-like memory, CD19⁺CD27⁻CD21⁻; resting memory, CD19⁺CD27⁺CD21⁺; activated mature, CD19⁺CD27⁺CD21⁻; plasmablasts, CD19⁺CD27⁺⁺CD38⁺⁺⁺; unswitched resting memory, CD19⁺CD21⁺CD27⁺IgD⁺; and switched resting memory, CD19⁺CD21⁺CD27⁺IgD⁻. The expressions of BR3 and TACI were also evaluated. The gating strategy is shown in Supplemental Fig. 1.

Muema D.M., Macharia G.N., Hassan A.S., Mwaringa S.M., Fegan G.W., Berkley J.A., Nduati E.W, & Urban B.C. (2015). Control of Viremia Enables Acquisition of Resting Memory B Cells with Age and Normalization of Activated B Cell Phenotypes in HIV-Infected Children. The Journal of Immunology Author Choice, 195(3), 1082-1091.

+ Open protocol

+ Expand

Immune Cell Phenotyping by Flow Cytometry

Check if the same lab product or an alternative is used in the 5 most similar protocols

Splenocytes, thymocytes, and peritoneal cavity cells were resuspended in PBS containing 1% FBS and Rat Anti-Mouse CD16/CD32 Mouse BD Fc Block (BD Biosciences). The following antibody combinations were used: anti-CD4-PE (BD Biosciences, clone GK1.5) and anti-CD8a-PerCP (BD Biosciences, clone 53–6.7); anti-IgD-PE (BD Biosciences, clone 11-26c.2a) and anti-IgM-APC (SouthernBiotech, clone 1B4B1); anti-CD5-APC (BD Biosciences, clone 53–7.3) and anti-IgM-PE (SouthernBiotech, clone 1B4B1); and anti-CD21-APC (BD Biosciences, clone 7G6) and anti-CD23-FITC (BD Biosciences, clone B3B4). Flow cytometry was performed with a BD FACSCaliber and Flojo software (Treestar).

Yu J.W., Hoffman S., Beal A.M., Dykon A., Ringenberg M.A., Hughes A.C., Dare L., Anderson A.D., Finger J., Kasparcova V., Rickard D., Berger S.B., Ramanjulu J., Emery J.G., Gough P.J., Bertin J, & Foley K.P. (2015). MALT1 Protease Activity Is Required for Innate and Adaptive Immune Responses. PLoS ONE, 10(5), e0127083.

+ Open protocol

+ Expand

Immunostaining and Flow Cytometry Protocol

Check if the same lab product or an alternative is used in the 5 most similar protocols

Primary antibodies used for confocal microscopy were: anti-F-actin (TRITC-phalloidin—Sigma), anti-alpha tubulin (clone DM1A, Sigma-aldrich), anti-pTyr-AF647 (clone PY99, Santa Cruz Biotechnology), anti-IFN-gamma (clone AN-18, BD Biosciences). Secondary antibodies used for confocal microscopy were: anti-rat AF568 (Molecular Probes) and anti-mouse AF594 (Molecular Probes). Antibodies used for flow cytometry were: anti-TCR DO11.10 PE (clone KJ1.26, MBL-medical and biological lab), anti-CD4 AF405 (clone RM4-5, Invitrogen), anti-CD80 PB (clone 16-10A1, BioLegend), anti-CD86 PE-Cy7 (clone PO3, BioLegend), anti-CD54 (ICAM1) APC (clone YN1/1.7.4, BioLegend), anti-CD40 PE-Cy7 (clone 3/23, BioLegend), anti-CD48 APC (clone HM48-1, BioLegend), anti-MHC-II PB (clone M5/114.15.2, BioLegend), anti-CD19 APC-H7 (clone 1D3, BD Biosciences), anti-CD21 APC (clone 7G6, BD Biosciences), anti-CD23 PE (clone B3B4, BD Biosciences), anti-CD69 FITC (clone H1.2F3, BioLegend), anti-CD95 PE (clone Jo2, BD Biosciences), anti-IFN-γ PE (clone XMG1.2, BD Biosciences). Primary antibodies used for western blot were: anti-EGFP (Clontech) and anti-actin (polyclonal, Sigma-Aldrich). Secondary antibodies used for western blot were anti-rabbit (GE Healthcare) and anti-mouse (Jackson Immunoresearch) antibodies, conjugated with horse radish peroxidase (HRP).

Fontinha D., Lopes F.B., Marques S, & Simas J.P. (2015). Murid Gammaherpesvirus Latency-Associated Protein M2 Promotes the Formation of Conjugates between Transformed B Lymphoma Cells and T Helper Cells. PLoS ONE, 10(11), e0142540.

+ Open protocol

+ Expand

Multiparametric Flow Cytometry Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols

The following antibodies were used: Anti-CD3 (Biolegend 100237), anti-CD11b, (Biolegend 101237), anti-IgM (Biolegend 406525), anti-CD19 (BD Biosciences 561739), anti-CD95 (BD Biosciences 561856), anti-GL7 (BD Biosciences 553666), anti-CD21 APC (BD Biosciences 561770), anti-CD138 (BD Biosciences 553714), Horseradish peroxidase conjugated anti-mouse Light Chain Specific goat polyclonal antibody (Jackson Immunoresearch #115-035-174), anti-IgG1 (BD Biosciences 560089), and for histology anti-CD45R/B220 (Bio-Rad, MCA1258G) and anti-CD138 (Biolegend 142502).

McBride K.M., Kil H., Mu Y., Plummer J.B., Lee J., Zelazowski M.J., Sebastian M., Abba M.C, & Aldaz C.M. (2019). Wwox Deletion in Mouse B Cells Leads to Genomic Instability, Neoplastic Transformation, and Monoclonal Gammopathies. Frontiers in Oncology, 9, 517.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!