A Chinese hamster ovary (CHO) cell line stably
expressing hM1 was obtained from Wyeth Research (Princeton, NJ). 96-Well
round-bottom and white 96-well plates were purchased from ThermoFisher
and from Greiner Bio One, Germany. Dulbecco’s modified Eagle’s
medium (DMEM) and phosphate-buffered saline (PBS) were from Sigma
(Schnelldorf, Germany). Leibovitz’s L-15 medium (L-15) and
Hank’s balanced salt solution (HBSS) were from ThermoFisher
(Dreieich, Germany). Fetal calf serum (FCS), trypsin, and geneticin
(G418) were from Merck Biochrom (Berlin, Germany).d -Luciferin
was purchased as a potassium salt from Pierce (ThermoFisher, Dreieich,
Germany) and was dissolved in HBSS at a concentration of 400 mM. Puromycin
was obtained from Invivogen (Toulouse, France). [3H]N-Methylscopolamine, 250 μCi (9.25 MBq), was purchased from
PerkinElmer (Rodgau, Germany) and polyethylenimine solution (1%, PEI)
from Sigma (Schnelldorf, Germany). For chromatographic applications
(HPLC, LC–MS), deionized water produced by means of a Milli-Q
system (Merck, Darmstadt, Germany) was used. HPLC grade and LC–MS
grade solvents were from Sigma-Aldrich (Munich, Germany). Unless stated
otherwise, all chemicals were purchased from Sigma-Aldrich (Schnelldorf,
Germany), VWR (Darmstadt, Germany), and TCI (Eschborn, Germany) and
were used without prior purification.
expressing hM1 was obtained from Wyeth Research (Princeton, NJ). 96-Well
round-bottom and white 96-well plates were purchased from ThermoFisher
and from Greiner Bio One, Germany. Dulbecco’s modified Eagle’s
medium (DMEM) and phosphate-buffered saline (PBS) were from Sigma
(Schnelldorf, Germany). Leibovitz’s L-15 medium (L-15) and
Hank’s balanced salt solution (HBSS) were from ThermoFisher
(Dreieich, Germany). Fetal calf serum (FCS), trypsin, and geneticin
(G418) were from Merck Biochrom (Berlin, Germany).
was purchased as a potassium salt from Pierce (ThermoFisher, Dreieich,
Germany) and was dissolved in HBSS at a concentration of 400 mM. Puromycin
was obtained from Invivogen (Toulouse, France). [3H]N-Methylscopolamine, 250 μCi (9.25 MBq), was purchased from
PerkinElmer (Rodgau, Germany) and polyethylenimine solution (1%, PEI)
from Sigma (Schnelldorf, Germany). For chromatographic applications
(HPLC, LC–MS), deionized water produced by means of a Milli-Q
system (Merck, Darmstadt, Germany) was used. HPLC grade and LC–MS
grade solvents were from Sigma-Aldrich (Munich, Germany). Unless stated
otherwise, all chemicals were purchased from Sigma-Aldrich (Schnelldorf,
Germany), VWR (Darmstadt, Germany), and TCI (Eschborn, Germany) and
were used without prior purification.