Triton x 100 bp 151

Manufactured by Thermo Fisher Scientific

Triton X-100 (BP-151) is a non-ionic surfactant commonly used in various laboratory applications. It is a clear, viscous liquid with a hydrophilic polyethylene oxide chain and a hydrophobic hydrocarbon group. This product is widely used as a detergent, emulsifier, and solubilizing agent in biochemical, cell biology, and molecular biology procedures.

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Lab products found in correlation

Ultrapure water system, by Merck Group (3 mentions) Phosphate buffered saline (pbs), by Avantor (1 mentions) β mercaptoethanol m3148, by Merck Group (1 mentions) Premixed 10 tris glycine sds electrophoresis buffer, by Bio-Rad (1 mentions) Phosphate buffered saline ph 7, by Thermo Fisher Scientific (1 mentions) Bovine serum albumin bsa a9418, by Merck Group (1 mentions) Gelslick 50640, by Lonza (1 mentions) Gelbond pag film for acrylamide gels, by Lonza (1 mentions) Triton x 100, by Merck Group (1 mentions) Sodium deoxycholate, by Merck Group (1 mentions) Fitc isomer 1, by Thermo Fisher Scientific (1 mentions) C acrylamide bis acrylamide, by Merck Group (1 mentions)

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Triton X-100 (995 citations) Triton-X (74 citations) Triton (14 citations) Triton-100 (6 citations)

5 protocols using triton x 100 bp 151

Functionalized Acrylamide Gel Electrophoresis

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All DNA oligomers were purchased from Integrated DNA Technologies (Coralville, IA, USA), including oligomers with 5′ acrydite groups for incorporation into the gel matrix (see Table S3 for all DNA sequences). dATP (R0141), dGTP (R0161), and dCTP (R0151) were purchased as 100 mM stock solutions from Thermo Fisher Scientific (Waltham, MA, USA). Alexa Fluor aha dUTP (A32763), SYBR Gold nucleic acid stain (S11494), and Triton X-100 (BP-151) were also purchased from Thermo Fisher. Tetramethylethylenediamine (TEMED, T9281), ammonium persulfate (APS, A3678), 3-(trimethoxylsilyl)propyl methacrylate (440159), dichlorodimethylsilane (440272), and 30%T/3.3%C acrylamide/bis-acrylamide (29:1) (A3574) were purchased from Sigma-Aldrich (St. Louis, MO, USA). N-[3-[(3-Benzoylphenyl)formamido]propyl] methacrylamide (BPMAC) was custom synthesized by Pharm-Agra Laboratories (Brevard, NC, USA). De-ionized water (18.2 MΩ) was obtained using an Ultrapure water system from Millipore. TE and TBE buffers were prepared according to standard protocols.³⁷

Moss A.C, & Herr A.E. (2020). In-gel fluorescence detection by DNA polymerase elongation. APL Bioengineering, 4(4), 046104.

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Lentiviral Transfection and GFP Protein Analysis

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Acetic acid (A6283), 3-(trimethoxysilyl)propyl methacrylate (662275), 30% T, 3.4% C acrylamide/bis-acrylamide (29:1) (A3574), N,N,N′,N′-tetramethylethylenediamine (TEMED, T9281), bovine serum albumin (BSA, A7030), ammonium persulfate (APS, A3678), sodium deoxycholate (D6750), and sodium dodecyl sulfate (SDS, L4509) were purchased from Sigma-Aldrich. Triton X-100 (BP-151) was attained from ThermoFisher Scientific. Tris-buffered saline with tween (20× TBST, 281695) was acquired from Santa Cruz Biotechnology. Premixed 10× Tris/glycine electrophoresis buffer (25 mM Tris, pH 8.3; 192 mM glycine) was procured from BioRad. Phosphate buffered saline (10× PBS, 45001–130) was purchased from VWR International. Deionized water (18.2 MΩ) was obtained using an Ultrapure water system from Millipore. N-[3-[(3-Benzoylphenyl)-formamido]propyl] methacrylamide (BPMAC) was custom synthesized by PharmAgra Laboratories. Lentiviral infection (multiplicity of 10) was performed to produce U373 MG cells expressing Turbo GFP, which were generously provided by Dr. Ching-Wei Chang in Prof. S. Kumar’s Laboratory. Rabbit anti-Turbo GFP antibodies (PA5-22688) were acquired from Pierce Antibody Products. Donkey antirabbit Alexa-Fluor 647-labeled secondary antibodies (A31573) were procured from Invitrogen. Recombinant Turbo GFP (FP552) was obtained from Evrogen.

Vlassakis J, & Herr A.E. (2015). Effect of Polymer Hydration State on In-Gel Immunoassays. Analytical chemistry, 87(21), 11030-11038.

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Synthesis and Characterization of BPMAC Compound

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Daunomycin (D8809-1MG), tetramethylethylenediamine
(TEMED, T9281), ammonium persulfate (APS, A3678), β-mercaptoethanol
(M3148), and 30%T, 2.7%C acrylamide/bis-acrylamide (37.5:1) (A3699)
were purchased from Sigma-Aldrich. Triton X-100 (BP-151) was purchased
from ThermoFisher Scientific. Premixed 10× Tris/glycine/SDS electrophoresis
buffer (25 mM Tris, pH 8.3; 192 mM glycine; 0.1% SDS) was purchased
from BioRad. Deionized water (18.2 MΩ) was obtained using an
Ultrapure water system from Millipore. N-[3-[(3-Benzoylphenyl)formamido]propyl]
methacrylamide (BPMAC) was custom synthesized by PharmAgra Laboratories.
Note that the BPMAC used in the previous studies was synthesized in-house^{17 (link),18 (link)} and is a positional isomer (para-form, N-[3-[(4-benzoylphenyl)formamido]propyl]
methacrylamide) of the BPMAC used in the present study.

Kang C.C., Lin J.M., Xu Z., Kumar S, & Herr A.E. (2014). Single-Cell Western Blotting after Whole-Cell Imaging to Assess Cancer Chemotherapeutic Response. Analytical Chemistry, 86(20), 10429-10436.

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Biomaterial Surface Functionalization Protocol

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Tetramethylethylenediamine (TEMED; T9281), ammonium persulfate (APS; A3678), β-mercaptoethanol (M3148), 30%T/2.7%C acrylamide/bis-acrylamide (37.5:1) (A3699), bovine serum albumin (BSA; A9418), Tyrode’s solution (T1788), trypsin 10× (59427C), Accutase (A6964), and 3-(trimethoxylsilyl)propyl methacrylate (440159) were purchased from Sigma-Aldrich. Triton X-100 (BP-151) and phosphate-buffered saline (PBS; pH 7.4; 10010023) were purchased from Thermo Fisher Scientific. Premixed 10× tris-glycine EP (electrophoresis) buffer [25 mM tris (pH 8.3), 192 mM glycine] was purchased from Bio-Rad. Tris-buffered saline with Tween-20 (TBST) was prepared from 20× TBST (sc-24953, Santa Cruz Biotechnology). Deionized water (18.2 megohms) was obtained using an ultrapure water system from Millipore. Alexa 555–labeled BSA (A34786) was purchased from Invitrogen. N-[3-[(3-benzoylphenyl)formamido]propyl] methacrylamide (BPMAC) was custom synthesized by Pharm-Agra Laboratories (Brevard, NC). GelSlick (50640) and Lonza GelBond PAG Film for Acrylamide Gels (BMA54746) were purchased from Lonza.

Rosàs-Canyelles E., Modzelewski A.J., Geldert A., He L, & Herr A.E. (2020). Assessing heterogeneity among single embryos and single blastomeres using open microfluidic design. Science Advances, 6(17), eaay1751.

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Polyacrylamide Gel Electrophoresis Reagents

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2,2-Azobis[2-methyl-N-(2-hydroxyethyl) pro-pionamide] (VA-086, 1% (w/v), photoinitiator) was purchased from Waco Chemical (Richmond, VA). Tetramethy-lethylenediamine (TEMED, T9281), ammonium persulfate (APS, A3678), β-mercaptoethanol (M3148), and a polymer precursor solution composed of 30% T and 2.7% C acrylamide/bis-acrylamide (37.5:1, A3699) were purchased from Sigma-Aldrich (St. Louis, MO). Triton X-100 (BP-151) was purchased from Thermo Fisher Scientific (Waltham, MA). Premixed 10× Tris/glycine/SDS-PAGE buffer (25 mM Tris, pH 8.3; 192 mM glycine; 0.1% SDS) was purchased from BioRad (Hercules, CA). Deionized water (18.2 MΩ) was obtained using an Ultrapure water system (Millipore, Burlington, MA). The cell lysis and PAGE buffer contained 0.5% SDS, 0.1% (v/v) Triton X-100, and 0.25% sodium deoxycholate (D6750, Sigma-Aldrich) in 12.5 mM Tris and 96 mM glycine (pH 8.3, 0.5× from a 10× stock; 161-0734, Bio-Rad, Hercules, CA). FITC (Isomer-I) used for polyacrylamide-gel imaging was from Invitrogen (Carlsbad, CA).

Pan Q., Yamauchi K.A, & Herr A.E. (2018). Controlling Dispersion during Single-Cell Polyacrylamide-Gel Electrophoresis in Open Microfluidic Devices. Analytical chemistry, 90(22), 13419-13426.

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