600 pump controller module

The amount of CUR in solution before and after dialysis was measured on solutions composed of 100 μL of liposomes suspension and 500 μL of CH 3 OH by HPLC measurements using a chromatographic system (Waters Corporation, Milford, MA) consisting of a Model 600 pump, a 600 pump controller module, a 717 Plus auto-sampler, and a 996 photodiode array detector. The E.E. for each formulation was evaluated by the ratio between the area of the peak corresponding to each CUR component before and after dialysis. The stability of CUR was evaluated by comparing the area of the peak corresponding to each CUR soon after the dialysis and after 3 h of incubation at room temperature in PBS buffer and in borax/boric acid buffer at pH = 8.6. The E.E. of each formulation was also evaluated by recording fluorescence (λ exc =425 nm, λ em =485 nm) spectra on a Perkin Elmer LS 50 spectrofluorimeter spectra before and after dialysis to remove free CUR. The determination of each E.E. was performed exploiting a calibration line (5 points) using the Sigma Aldrich standards of the three active principles contained in CUR in the range of the concentration tested.