Cyan adp

Manufactured by Tree Star

Sourced in United States

The CyAn ADP is a flow cytometer designed for detailed analysis of cell samples. It features a high-performance optical system and advanced data acquisition capabilities to provide accurate and reliable results. The core function of the CyAn ADP is to facilitate the detection and analysis of cells, particles, and other biological entities within a sample.

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Lab products found in correlation

Facs canto, by Tree Star (1 mentions) Moflo xdp, by Tree Star (1 mentions) Fix perm buffer, by Thermo Fisher Scientific (1 mentions)

Spelling variants of this product

CyAN ADP flow cytometer (7 citations) CyAn ADP cytometer (4 citations)

5 protocols using cyan adp

Immunophenotyping of Mouse and Human Leukemia Cells

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Primary leukemia cells from premorbid mice were harvested and treated with ammonium chloride potassium (ACK) buffer to lyse mature red cells. Cells were then blocked with anti-mouse CD16/CD32 (FcBlock) and stained with an antibody cocktail containing 145-2C11 (CD3e), GK1.5 (CD4), 53-6.7 (CD8), RB6-8C5 (Ly-6G/Gr-1), M1/70 (CD11b/Mac-1), TER119 (Ly-76/TER119), 6B2 (CD45R/B220), 2B8 (CD117/c-Kit), D7 (Ly-6A/E/Sca-1) and A2F10 (CD135/Flt3). For human MV-4-11, cells were harvested, blocked and stained with BV10A4H2 for human CD135/FLT3. All antibodies were purchased from eBioscience or Biolegend. Flow cytometric analyses were performed on FACSCanto, MoFlo XDP or CyAn ADP flow analyzers, and data were analyzed with FlowJo software (Tree Star Inc.)

Hattori A., McSkimming D., Kannan N, & Ito T. (2017). RNA binding protein MSI2 positively regulates FLT3 expression in myeloid leukemia. Leukemia research, 54, 47-54.

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Intracellular IFN-γ and IL-17 Detection in CD4+ T Cells

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For detection of intracellular interferon-γ (IFN-γ) and IL-17 in CD4⁺ T cells, splenic and pancreatic lymph node cells were stimulated with phorbol myristic acid (PMA) (50 ng/mL) and ionomycin (500 ng/mL) for 4 hour in the presence of brefeldin A (10 μg/mL) and then stained with peridinin-chlorophyll-protein (PerCP)-cy5.5–conjugated anti-CD4 (RM4–5) and fluorescein isothiocyanate (FITC)–conjugated anti-CD3 (145–2C11) antibodies (all from BD Biosciences) for cell surface staining. Subsequently, the cells were fixed and permeabilized with Fix/Perm buffer (eBioscience) and incubated with allophycocyanin-conjugated anti-IFN-γ and PE-conjugated anti–IL-17 Abs (BD Biosciences) for intracellular staining. The samples were read using a Beckman Coulter CyAn ADP, and data were analyzed using FlowJo 10.0.8 software (TreeStar).

Ukah T.K., Cattin-Roy A.N., Davis G.E, & Zaghouani H. (2021). Formation of pancreatic β-cells from precursor cells contributes to the reversal of established type 1 diabetes. Cellular immunology, 364, 104360.

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Flow Cytometric Immunophenotyping Protocol

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Cell surface molecular expression and intracellular cytokine production were analysed using FCM. A total of 10 000 events (minimum) were acquired via a flow cytometer (Beckman‐Coulter CyAN ADP) with analysis using FlowJo software (Tree Star, Ashland, OR, USA). Cell surface staining was performed with flurochrome conjugated monoclonal antibodies for 30 min incubation. After fixation and permeabilisation, cells were stained for intracellular cytokines and nuclear transcription factors. Antibodies were purchased from Biolegend, USA or eBioscience, USA (Supplemental Table S4). Isotype‐matched IgG reagents were used as negative controls. Fluorescence‐activated cell sorting was performed according to the manufacturer's instructions.

Tao Y., Li Y., Zhang D., Xu L., Chen J., Sang Y., Piao H., Jing X., Yu M., Fu Q., Zhou S., Li D, & Du M. (2021). Decidual CXCR4+CD56brightNK cells as a novel NK subset in maternal–foetal immune tolerance to alleviate early pregnancy failure. Clinical and Translational Medicine, 11(10), e540.

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Multicolor Flow Cytometry Analysis of Whole Blood

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Multicolor flow cytometry analysis was performed on whole blood. Samples were stained with the following fluorochrome-conjugated antibodies: anti-CD14 Phycoerythrin Cyanine-7 (PECy7), anti-CD3 Allophycocyanin-H7 (APC-H7), anti-CD4 Pacific Blue (PB), anti-CD8 Fluorescein (FITC), anti-CD8 (PECY7), CD45RA Phycoerythrin (PE), CD45RA PECY5, CCR7 Allophycocyanin (APC), anti-CD19 (APC), anti-CD19 Peridinin-Chlorophyll-Protein (PERCP), anti-CD27 (PE), CD10 PE-Cy7, CD21 FITC, CD27 APC-Cy7, CD95 e450, FcLR4 PE, CD57 FITC, PD-1 APC, and PD-1 PE (Supplementary 2–5 Gating Strategy and Table 2). Samples were acquired on Beckman Coulter Cyan ADP and analyses were performed using FlowJo Version 7.6.5 and 10.5 software (TreeStar).

Afran L., Jambo K.C., Nedi W., Miles D.J., Kiran A., Banda D.H., Kamg’ona R., Tembo D., Pachnio A., Nastouli E., Ferne B., Mwandumba H.C., Moss P., Goldblatt D., Rowland-Jones S., Finn A, & Heyderman R.S. (2022). Defective Monocyte Enzymatic Function and an Inhibitory Immune Phenotype in Human Immunodeficiency Virus-Exposed Uninfected African Infants in the Era of Antiretroviral Therapy. The Journal of Infectious Diseases, 226(7), 1243-1255.

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Cell Cycle Profiling of MEFs

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MEFs cultured in the presence or absence of 10% FCS were labeled with BrdU (10 μM) for 1 hr, fixed in 70% EtOH and stained with Propidium Iodide (PI). Data acquisition was performed on a Beckman Coulter Cyan ADP and data analysis (cell cycle) was performed using FlowJo software version 7.6.1 (Tree Star, Ashland, OR, USA).

Benedict B., van Harn T., Dekker M., Hermsen S., Kucukosmanoglu A., Pieters W., Delzenne-Goette E., Dorsman J.C., Petermann E., Foijer F, & te Riele H. (2018). Loss of p53 suppresses replication-stress-induced DNA breakage in G1/S checkpoint deficient cells. eLife, 7, e37868.

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