A lyophilized stock of the organisms was obtained from the American Type Culture Collection (ATCC). The commensal bacteria Lactobacillus rhamnosus (ATCC 7469), Lactococcus lactis (ATCC 11454), Enterococcus faecium (ATCC 6057), and the microorganisms causing opportunistic infections Escherichia coli (ATCC 25922) as well as Candida albicans (ATCC 64548) and Candida glabrata (ATCC 15126) isolates were used in the present study. All isolates were previously purchased in MicroSwab form from Merck (Warsaw, Poland). The bacterial and fungal isolates were freshly cultivated on Tryptone Soy Broth (TSB) (Merck, Poland) before the experiments and also stored in TSB with 20% glycerol at − 70 °C until needed. The bacteria were routinely cultured on TSB (pH 7.3) for 2 days at 37 °C and the fungi were cultured on Sabouraud broth (pH 5.8) for 6 days at 24.5 °C. Sabouraud broth contains mycological peptone (10 g/L), glucose (20 g/L). All of the microorganisms were incubated in tightly closed polypropylene 12 mL Falcon tubes, in anaerobic conditions.
Lactococcus lactis
Manufactured by American Type Culture Collection
Lactococcus lactis is a lactic acid bacterium commonly used in the production of dairy products. It is a Gram-positive, non-spore-forming, catalase-negative, and homofermentative bacterium. Lactococcus lactis is known for its ability to ferment various carbohydrates and produce lactic acid as the primary end-product.
Automatically generated - may contain errors
Lab products found in correlation
Tryptic soy broth (tsb), by Merck Group (1 mentions)
Tryptone soy broth (tsb), by Merck Group (1 mentions)
Lactobacillus rhamnosus, by American Type Culture Collection (1 mentions)
Escherichia coli, by American Type Culture Collection (1 mentions)
Bacteroides ovatus, by American Type Culture Collection (1 mentions)
Lactobacillus paracasei, by American Type Culture Collection (1 mentions)
Lactobacillus acidophilus atcc, by American Type Culture Collection (1 mentions)
Bifidobacterium breve, by American Type Culture Collection (1 mentions)
Clostridium perfringens, by American Type Culture Collection (1 mentions)
Lactobacillus reuteri, by American Type Culture Collection (1 mentions)
Bacteroides fragilis, by American Type Culture Collection (1 mentions)
Ruminococcus gnavus, by American Type Culture Collection (1 mentions)
2 protocols using lactococcus lactis
1
Microbial Isolate Cultivation and Storage
2
Culturing Diverse Anaerobic Bacteria
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Bacteria strains used are either purchased from ATCC (Lactobacillus acidophilus ATCC® 4356™, Lactobacillus paracasei ATCC® BAA-52™, Lac-tobacillus reuteri ATCC® 23272™, Lactococcus lactis ATCC® 19435™, Bifidobacterium breve ATCC® 15700™, Bifidobacterium longum subsp. longum ATCC® 15707™, Clostridium perfringens ATCC® 13124™, Ruminococcus gnavus 35913™) or obtained from the Sonnenburg lab (Bacteroides fragilis NCTC 9343, Bacteroides finegoldii DSM 17565, Bacteroides dorei DSM17855, Bacteroides ovatus ATCC 8483, Lactobacillus plantarum ATCC BAA-793, Lactobacillus rhamnosus ATCC 53103).
Lactobacilli MRS Agar plate (Hardy Diagnostic, USA) was used to culture L. acidophilus, L. paracasei, L. reuteri, L. plantarum, L. rhamnosus, L. lactis. Blood Agar plate (Hardy Diagnostic, USA) was used to culture B. breve, B. longum, C. perfringens, R. gnavus. BHI agar plate (Hardy Diagnostic, USA) was used to culture B. finegoldii, B. dorei, B. ovatus, B. fragilis.
Each bacterial strain was inoculated onto the respective culture plates under sterile conditions. The plates were then placed in an air-tight gas pouch containing one pack of BD Difco™ GasPak™ EZ Gas Generating System (ThermoFisher B260683, USA) to create an anaerobic environment. The pouches were incubated at 37 °C for 48 hrs until harvest, allowing the bacteria to grow and develop.
Lactobacilli MRS Agar plate (Hardy Diagnostic, USA) was used to culture L. acidophilus, L. paracasei, L. reuteri, L. plantarum, L. rhamnosus, L. lactis. Blood Agar plate (Hardy Diagnostic, USA) was used to culture B. breve, B. longum, C. perfringens, R. gnavus. BHI agar plate (Hardy Diagnostic, USA) was used to culture B. finegoldii, B. dorei, B. ovatus, B. fragilis.
Each bacterial strain was inoculated onto the respective culture plates under sterile conditions. The plates were then placed in an air-tight gas pouch containing one pack of BD Difco™ GasPak™ EZ Gas Generating System (ThermoFisher B260683, USA) to create an anaerobic environment. The pouches were incubated at 37 °C for 48 hrs until harvest, allowing the bacteria to grow and develop.
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