The following monoclonal antibodies (mAbs) used: anti-rat-Nrp-PE, anti-mouse-PD-L1-PE (eBioscience, San Diego, CA), anti-mouse/rat CD28-PE (Santa Cruz, CA), anti-rat CD4-FITC, anti-rat CD25-PE, anti-rat Foxp3-PE-cyanine 5 (eBioscience, San Diego, CA, USA). After membrane and intracellular staining, cells were analyzed on FACS CantoII using the BD FACSDiva v8.0 software.
Anti rat foxp3 pe cyanine 5
Manufactured by Thermo Fisher Scientific
Sourced in United States
Anti-rat Foxp3-PE-cyanine 5 is a fluorescently-labeled antibody that binds to the Foxp3 transcription factor, which is a marker for regulatory T cells. It can be used for the identification and analysis of regulatory T cells in rat samples.
Automatically generated - may contain errors
2 protocols using anti rat foxp3 pe cyanine 5
1
Multicolor Flow Cytometry Analysis
2
Identification of Regulatory T Cells
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Cells were analyzed on a flow cytometer (BD Bioscience) according to a previously reported method [34 (link)]. For intracellular staining, cells were fixed and permeabilized with Cytofix/Cytoperm (BD PharMingen). The following mAbs were included: anti-rat CD25-PE, anti-rat CD4-FITC, and anti-rat Foxp3-PE-cyanine 5 (eBioscience, San Diego, CA, USA). As to the Foxp3 antibody, an isotype-matched control was used to determine the background. Tregs were identified as CD4+CD25+Foxp3+ triple-positive cells and were expressed as percentages in SC.
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