SJL/J male mice were injected intraperitoneally with two doses of 100 mg/kg ENU (Sigma-Aldrich, St Louis, MO) at 1 wk intervals. The treated males (G0) were crossed to females from the isogenic background to produce the first generation progeny (G1). The 7-wk-old G1 progeny were bled and analyzed on an Advia 120 Automated Hematology Analyzer (Siemens, Berlin, Germany) to identify abnormal RBC count. A mouse carrying the MRI96570 or MRI95845 mutation was identified with an RBC “mean corpuscular volume” (MCV) value three SD lower than other G1 progeny. It was crossed with SJL/J mice to produce G2 progeny to test the heritability of the mutations and the dominant mode of inheritance. Mice that exhibited a low MCV (<48 fL) were selected for whole exome sequencing and genotyping.
Advia 120 automated hematology analyzer
Manufactured by Siemens
Sourced in Germany, United States
The Advia 120 Automated Hematology Analyzer is a laboratory equipment product designed to perform complete blood count (CBC) analysis. It is an automated system that can efficiently analyze and provide key hematological parameters, including red blood cell count, white blood cell count, and platelet count.
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Lab products found in correlation
2 protocols using advia 120 automated hematology analyzer
1
Identifying Hereditary Low RBC MCV Mutations
2
Laboratory Biomarkers of Chronic Fatigue
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The following laboratory results were recorded: white blood cell count, hemoglobin, glucose, liver function test (alanine transaminase, aspartate aminotransferase, and alkaline phosphatase), kidney function test (blood urea nitrogen-to-creatinine ratio), thyroid-stimulating hormone, electrolytes, total protein, albumin, globulin, urinalysis (hematuria and proteinuria), C-reactive protein, and erythrocyte sedimentation rate. These laboratory items were regarded as causes of fatigue based on CFS’s diagnosis criteria in the Center for Disease Control and Prevention [15 (link)]. The normal range of these items followed the standard of Chung-Ang University Hospital. ADVIA 120 automated hematology analyzer (Siemens, Tarrytown, NY, USA) and ADVIA 1650 Chemistry System (Siemens) were used.
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