The SNP A118G of OPRM1 was chosen on the basis of two criteria: 1) SNP of the opioid system that was previously reported as associated with suicide and depression; 2) minor allele frequencies above 5%. The Hardy-Weinberg equilibrium was respected.
DNA was collected at baseline by buccal swab. Genotyping was performed with a 5ʹ exonuclease assay (TaqMan, Life Technologies). Assay products were run on an applied Biosystem 7900HT Fast Real-Time PCR System (Life Technologies).
The success rate of SNP genotyping in our population was 95%. In each 96-well plate, four DNA samples were assessed in replicate (duplicate) to measure the reproducibility rate. The plate was genotyped again When a discrepancy in one of these replicates was observed. Overall, the replication rate was 99.9% for all genotyping runs.
DNA was collected at baseline by buccal swab. Genotyping was performed with a 5ʹ exonuclease assay (TaqMan, Life Technologies). Assay products were run on an applied Biosystem 7900HT Fast Real-Time PCR System (Life Technologies).
The success rate of SNP genotyping in our population was 95%. In each 96-well plate, four DNA samples were assessed in replicate (duplicate) to measure the reproducibility rate. The plate was genotyped again When a discrepancy in one of these replicates was observed. Overall, the replication rate was 99.9% for all genotyping runs.