CpG ODN 1826 (5ʹ-tccatgacgttcctgacgtt-3ʹ) and isotype control were purchased from InvivoGen (San Diego, CA, USA). Anti-OX40 (CD134) monoclonal antibody (clone OX86) and isotype control were purchased from BioXCell (Lebanon, NH, USA). Dulbecco’s modified Eagle’s medium (DMEM), fetal bovine serum (FBS), penicillin/streptomycin solution, phosphate-buffered saline (PBS) and TRIzol reagent were purchased from Invitrogen Life Technologies (Carlsbad, CA, USA). The Matrigel was purchased from Corning (Corning, NY, USA). The TUNEL Apoptosis Detection Kit was purchased from KeyGen BioTECH (Nanjing, Jiangsu, China). The T Cell Isolation Kit was purchased from Miltenyi Biotec (Bergisch Gladbach, Germany). Antibodies including CD45-PerCP-Cy5.5, CD3e-BV510, CD4-FITC, CD25-PE, OX40-APC, CD8a-FITC, CD11b-BV421, Gr-1-APC, F4/80-PE, CD8a-APC, CD44-PE-Cy7, CD62L-PE, IFN-γ-PE, and FoxP3-eFluor450 were purchased from BD Biosciences (San Jose, CA, USA) and Biolegend (San Diego, CA, USA).
Isotype control
Manufactured by InvivoGen
Sourced in United States
Isotype controls are antibodies that have the same immunoglobulin isotype as the primary antibody, but recognize an antigen not present in the sample. They are used to determine the specificity of the primary antibody and to assess the level of nonspecific binding.
Automatically generated - may contain errors
Lab products found in correlation
T cell isolation kit, by Miltenyi Biotec (1 mentions)
Cd11b bv421, by BD (1 mentions)
Isotype control, by BioXCell (1 mentions)
Ifn γ pe, by BD (1 mentions)
Cd62l pe, by BD (1 mentions)
Gr1 apc, by BD (1 mentions)
Ox40 apc, by BD (1 mentions)
Cd3e bv510, by BD (1 mentions)
Cd8a apc, by BD (1 mentions)
Cd25 pe, by BD (1 mentions)
F4 80 pe, by BD (1 mentions)
Cpg odn 1826, by InvivoGen (1 mentions)
Phosphate buffered saline (pbs), by Thermo Fisher Scientific (1 mentions)
Trizol reagent, by Thermo Fisher Scientific (1 mentions)
Cd44 pe cy7, by BD (1 mentions)
Tunel apoptosis detection kit, by Keygen Biotech (1 mentions)
Matrigel, by Corning (1 mentions)
Cd4 fitc, by BD (1 mentions)
Cd45 percp cy5, by BD (1 mentions)
Cd8a fitc, by BD (1 mentions)
2 protocols using isotype control
1
Immunomodulation of Tumor Microenvironment
2
Inhibition of TLR2 in Bone Marrow-Derived Mast Cells
Check if the same lab product or an alternative is used in the 5 most similar protocols
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2x105 BMMC/0.25 mL of complete medium were pre-incubated 30 minutes with 200 ng/mL of anti-TLR2 (clone: C9A12; IgG2a) or 200 ng/mL of isotype control (clone: T9C6; 1gG2a), both from Invivogen, USA. Afterwards, cells were stimulated with L.m MOI 100:1 and the bacterial load, production, degranulation, cytokines production and evaluation of phosphorylated proteins assays were performed as indicated above. The bacterial load was analyzed as endocytic activity and bactericidal activity, according to the following formula: endocytic activity = (CFU at 0 h/MOI added) *100 (%). Bactericidal activity = (100-(CFU at 24 h/CFU at 0 h) *100) (%) (32 (link)). To evaluate the efficiency of antibody-mediated TLR2 inhibition, 2x105 BMMC/0.25 mL were pre-incubated with different concentrations of anti-TLR2 (12.5, 25, 50, 100 y 200 ng/mL) for 30 min and then stimulated with the TLR2 agonist: Staphylococcus aureus peptidoglycan (PGN) (Sigma-Aldrich, USA) at 10 μg/mL for 24 h. Then, supernatants were collected for the detection of IL-6 by ELISA (Supplementary Figure 5
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