C57BL/6/J mice, Ly5.1 (B6.SJL-Ptprca Pepcb/BoyJ), Thy1.1 mice, OT-1 mice, and Alb-Cre (B6.Cg-Tg(Alb-cre)21Mgn/J) mice were purchased from Jackson Laboratories. Cd36−/− mice (Coburn et al., 2000 ), 10BiT mice (Maynard et al., 2007 (link)), P14 mice (Kaech et al., 2003 (link)), AST (Albumin-floxStop-SV40 large T antigen (TAG)) mice (Philip et al., 2017 (link)), and TCRTAG transgenic mice (B6.Cg-Tg(TcraY1,TcrbY1)416Tev/J) (Staveley-O’Carroll et al., 2003 (link)) have been previously described. P14 Cd36−/− mice were generated by crossing P14 mice with Cd36−/− mice. TCRTAG mice were crossed to Thy.1.1 mice to generate TCRTAG Thy.1.1 mice. AST mice were crossed to Alb-Cre mice to generate AST-Alb-Cre mice. Animals were housed in specific-pathogen-free facilities at the Salk Institute and all experimental studies were approved and performed in accordance with guidelines and regulations implemented by the Salk Institute Animal Care and Use Committee.
Ly5.1 b6 sjl ptprca pepcb boyj
Manufactured by Jackson ImmunoResearch
Sourced in Montenegro
Ly5.1 (B6.SJL-Ptprca Pepcb/BoyJ) is a laboratory mouse strain that serves as a genetic marker. It is commonly used in immunological research to identify and distinguish different cell types.
Automatically generated - may contain errors
Lab products found in correlation
Alb cre, by Jackson ImmunoResearch (1 mentions)
Ot 1 mice, by Jackson ImmunoResearch (1 mentions)
B6 cg tg alb cre 21mgn j mice, by Jackson ImmunoResearch (1 mentions)
C57bl 6j mice, by Jackson ImmunoResearch (1 mentions)
B6.129s2 cd8atm1mak j, by Jackson ImmunoResearch (1 mentions)
αpd l1, by BioXCell (1 mentions)
B6.129s7 ifn γtm1ts j, by Jackson ImmunoResearch (1 mentions)
C57bl 6, by Jackson ImmunoResearch (1 mentions)
3 protocols using ly5.1 b6 sjl ptprca pepcb boyj
1
Generation and Characterization of Transgenic Mice
2
Immunomodulation in Murine Ovarian Cancer Model
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Female C57BL/6, IFN-γ−/− (B6.129S7-IFNγtm1Ts/J), CD8−/− (B6.129S2-Cd8atm1Mak/J), Ly5.1 (B6.SJL-Ptprca Pepcb/BoyJ), and IL12R−/− (B6.129S1-Il12rb2tm1Jm/J) mice age 6-8 weeks were purchased from Jackson Laboratories, Bar Harbor, ME. For day 35 (D35) and day 42 (D42) experiments, 1 × 107 ID8-Muc16ecto tumor cells were injected intraperitoneally (i.p.) on D0 and animals were treated with CAR T cells on D35 or D42 respectively. Mice treated with αFasL blocking antibody (R&D systems) were treated with 250 μg/mouse of antibody on D41 after tumor inoculation. Animals treated with PBS/Clodronate liposomes (Cedarlane, Amsterdam, The Netherlands) were injected with 200 μL/mouse of either liposome on D38 and D40 after tumor injection. Pretreatment with a single dose of 250 μg/mouse of αPD-L1 (BioXcell, West Lebanon, NH) was performed on D41 after tumor inoculation. All injections were i.p. All mice were monitored for survival and were euthanized when showing signs of distress or significant ascites.
3
Generating Stat3 Hematopoietic Chimeras
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Recipient Ly5.1 [B6.SJL-Ptprca Pepcb/BoyJ (Jackson) at six wks of age were gamma irradiated with 900 rads, in split dose. Next, mice were injected retro-orbitally with two million bone marrow cells isolated from donor CD19cre/+Stat3f/ftdTomatostopf/f and Stat3f/ftdTomatostopf/f mice at a ratio of 50:50 WT:KO bone marrow for the first set of chimeras, or with five million bone marrow cells isolated from donor CD19cre/+Stat3f/ftdTomatostopf/f and CD19cre/+ mice at a ratio of 30:70 WT:KO bone marrow for the second set of chimeras. Mice were treated with acidified water two weeks prior to and after irradiation. Verification of chimerism was performed six weeks post transfer through collection of blood by submandibular bleed, followed by flow cytometry. The mean reconstitution ratio was 66% WT: 34% KO cells for the first set of chimeras and 39% WT: 61% KO for the second set of chimeras.
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