Bio tek el x 800 elisa reader

Serum was used for the determination of various lipid marker parameters such as triglycerides (TG) [18 (link)], total cholesterol (TCh) [19 (link)] and high density lipoprotein cholesterol (HDL-C) [20 (link)] using Crescent Diagnostics kits, KSA. The value of low density lipoprotein cholesterol (LDL-C) was calculated according to the Friedwald equation [21 (link)]. Lactate dehydrogenase (LDH) was estimated in serum by simple colorimetric method using Abcam's assay kit [22 (link)]. NAD is reduced to NADH by LDH, which further interacts with specific probe to produce colour. The development of colour formation was estimated at 450 nm. Creatine kinase MB was estimated by simple in vitro sandwich ELISA using Abcam's assay kit. Standards and test samples were analyzed by binding with immobilized antibody which captures CKMB present in the test sample. The development of colour formation was estimated at 450 nm using Bio Tek EL X 800 ELISA reader. Cardiac troponin I was quantitatively measured in serum by simple in vitro sandwich ELISA. This method utilizes an affinity tag antibody and a reporter conjugated antibody which immunocapture the standard and sample present in solution. The end reaction was measured by the development of colour formation which was estimated at 450 nm using Bio Tek EL X 800 ELISA reader.

RayBiotech Human Fetuin A ELISA Kit, RayBiotech Human ACAN ELISA Kit, RayBiotech Human FLRG ELISA Kit, RayBiotech Human FSTL1 ELISA Kit, RayBiotech Human IGFBP3 ELISA Kit, RayBiotech Human TSP5 ELISA Kit, and RayBiotech Human MMP-13 ELISA Kit were used (RayBiotech Inc.). Biotek Elx800 ELISA Reader, Biotek Elx50 Microplate Washer (Biotek Instruments Inc., Burlington, VM, USA), and Sigmalplot 12.0 Analysis Software (Systat Software Inc., San Jose, CA, USA) were also used.