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Cathepsin l epr8011

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Cathepsin L (EPR8011) is a recombinant protein product. It is a cysteine protease that is involved in the degradation of proteins in lysosomes.

Automatically generated - may contain errors

Lab products found in correlation

Axio imager 2, by Zeiss (2 mentions) Apotome, by Zeiss (2 mentions) Zen software, by Zeiss (2 mentions) Cd107a 1d48, by BioLegend (2 mentions) Biotin anti rabbit, by Vector Laboratories (2 mentions) Streptavidin dylite 550, by Thermo Fisher Scientific (2 mentions)

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Cathepsin L (4 citations)

2 protocols using cathepsin l epr8011

1

Immunohistochemistry of Lysosomal Proteins

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LNs were enzymatically digested, depleted of CD45+ cells, stained with 10.1.1 extracellularly and CD107a (1D48, BioLegend), cathepsin L (EPR8011, Abcam), biotin-anti-rabbit (Vector labs) and streptavidin Dylite 550 (Thermo Scientific) intracellularly before being cytospun onto slides and mounted with Slow Fade Gold with DAPI. Images were taken using an Axio Imager 2 with Apotome (Carl Zeiss), and brightness and contrast were adjusted using Zen software (Carl Zeiss).
Rouhani S.J., Eccles J.D., Riccardi P., Peske J.D., Tewalt E.F., Cohen J.N., Liblau R., Mäkinen T, & Engelhard V.H. (2015). Roles of lymphatic endothelial cells expressing peripheral tissue antigens in CD4 T-cell tolerance induction. Nature Communications, 6, 6771.
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2

Immunofluorescent Analysis of LN Cells

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LN were enzymatically digested, depleted of CD45+ cells, stained with 10.1.1 extracellularly and CD107a (1D48, Biolegend), cathepsin L (EPR8011, Abcam), biotin-anti-rabbit (Vector labs) and streptavidin Dylite 550 (Thermo Scientific) intracellularly prior to being cytospun onto slides and mounted with Slow Fade Gold with DAPI. Images were taken using an Axio Imager 2 with Apotome (Carl Zeiss), and brightness and contrast were adjusted using Zen software (Carl Zeiss).
Rouhani S.J., Eccles J.D., Riccardi P., Peske J.D., Tewalt E.F., Cohen J.N., Liblau R., Mäkinen T, & Engelhard V.H. (2015). Roles of lymphatic endothelial cells expressing peripheral tissue antigens in CD4 T-cell tolerance induction. Nature Communications, 6, 6771.
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