Rabbit anti gapdh 2118

Manufactured by Cell Signaling Technology

Sourced in United States, United Kingdom

Rabbit anti-GAPDH (#2118) is a primary antibody that specifically recognizes the glyceraldehyde-3-phosphate dehydrogenase (GAPDH) protein. GAPDH is a ubiquitous enzyme involved in the glycolytic pathway.

Automatically generated - may contain errors

Lab products found in correlation

Cell counting kit 8 cck 8, by Beyotime (3 mentions) Trizol, by Thermo Fisher Scientific (1 mentions) Cell counting kit 8 (cck8), by Beyotime (1 mentions) 4 6 diamidino 2 phenylindole (dapi), by Keygen Biotech (1 mentions) Bicinchoninic acid bca protein assay kits, by Dingguo (1 mentions) Rabbit anti flag, by Merck Group (1 mentions) Ap307p, by Merck Group (1 mentions) Mouse anti brdu, by Thermo Fisher Scientific (1 mentions) F7425, by Merck Group (1 mentions) Mouse anti k8, by Santa Cruz Biotechnology (1 mentions) Mouse anti β actin ac 15, by Merck Group (1 mentions) Pa522819, by Thermo Fisher Scientific (1 mentions) Rabbit anti timp3 ab39184, by Abcam (1 mentions) Andrographolide andro, by Merck Group (1 mentions) Andro 365645, by Merck Group (1 mentions) P8765, by Merck Group (1 mentions) Rabbit anti ki67 ab15580, by Abcam (1 mentions) Tom20 sc 11415, by Santa Cruz Biotechnology (1 mentions) Anti vdac ab15895, by Abcam (1 mentions) Anti tim23 611222, by BD (1 mentions)

Close spelling variants of this product

GAPDH (5572 citations) Anti-GAPDH (1707 citations) Rabbit anti-GAPDH (354 citations) GAPDH (2118) (89 citations) Anti-GAPDH (2118) (27 citations)

8 protocols using rabbit anti gapdh 2118

DAPI-Based HIF-1α Modulation Assay

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DAPI (4′,6‐diamidino‐2‐phenylindole) (KGR0001) was purchased from KeyGen Biotech (Nanjing, China). TRIzol was obtained from Invitrogen (CA, USA). Recombinant human IL‐6 (I1395) was obtained from Sigma‐Aldrich (St. Louis, MO, USA). The siRNA duplex for HIF‐1α was synthesized by Genepharma Co., Ltd. (Shanghai, China), as described elsewhere 18. Cell Counting Kit‐8 (C0037) was obtained from Beyotime (Jiangsu, China). Mouse IL‐6 enzyme‐linked immunosorbent assay (ELISA) Kit (EK0411) was obtained from Boster (Wuhan, China). The following primary antibodies were used for western blotting assay: rabbit anti‐HIF‐1α (PB0245, diluted to 1:500, Boster) and rabbit anti‐GAPDH (2118s, diluted to 1:2000, Cell Signaling Technology, Inc., Danvers, MA, USA).

Dai J., Sun C., Yao Z., Chen W., Yu L, & Long M. (2016). Exposure to concentrated ambient fine particulate matter disrupts vascular endothelial cell barrier function via the IL‐6/HIF‐1α signaling pathway. FEBS Open Bio, 6(7), 720-728.

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Comprehensive Immunohistochemistry Protocol

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Goat anti-rabbit IgG (98164S), rabbit anti-Iba-1 (17198S), rabbit anti-NeuN (24307S), rabbit anti-COX2 (12282S), and rabbit anti-GAPDH (2118S) were obtained from Cell Signaling Technology (Danvers, MA, United States). Rabbit anti-BDNF (ab108319), rabbit anti-IL6 (ab259341), rabbit anti-IL1β (ab254360), and rabbit anti-TNF-α (ab205587) were purchased from Abcam (Cambridge, MA, United States). 2,3,5-triphenyltetrazolium chloride (TTC, purity >98.0%) was purchased from Sigma-Aldrich (St. Louis, MO, United States). Sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and bicinchoninic acid (BCA) protein assay kits were purchased from Dingguo Changsheng Biotechnology (Beijing, China). Tris-buffered saline (TBS), transfer buffer (pH 8.4), and running buffer (pH 8.4–8.6) were purchased from Servicebio (Wuhan, China). Other reagents were provided by other suppliers.

Liao Y., Wang J., Guo C., Bai M., Ju B., Ran Z., Hu J., Yang J., Wen A, & Ding Y. (2022). Combination of Systems Pharmacology and Experimental Evaluation to Explore the Mechanism of Synergistic Action of Frankincense-Myrrh in the Treatment of Cerebrovascular Diseases. Frontiers in Pharmacology, 12, 796224.

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Antibody Panel for KSHV Analysis

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Antibodies used in this study include rabbit anti-Syncytin-1 (for western blot, PA522819; Invitrogen), rabbit anti-Syncytin-1 (for flow cytometry, PA522819; Invitrogen), mouse anti-bZIP (sc69797; Santa Cruz), mouse anti-K8.1 (sc65446; Santa Cruz), rabbit anti-KSHV RTA (a gift from Yoshihiro Izumiya at University of California Davis), mouse anti-β-actin (AC-15; Sigma), rabbit anti-GAPDH (2118S; Cell Signaling), mouse anti-EA-D (MAB8186; EMD), mouse IgG1 negative isotype (CBL610; EMD Millipore), mouse anti-ZEBRA (a gift from Paul Farrell at Imperial College London, London, United Kingdom), rabbit anti-FLAG (F7425; Millipore), rat anti-EBNA2 (MABE8; Millipore), mouse anti-BrdU (555627; Thermo Fisher Scientific), mouse anti-CD19-APC (MABF197; Sigma-Aldrich), mouse anti-CD20-PE (MABF1635; Sigma-Aldrich), mouse IgG1-APC isotype (550824; BD Pharmingen), rat IgG2a isotype (MABF1077Z; EMD Millipore), horseradish peroxidase (HRP)-conjugated goat anti-mouse IgG (H + L) (AP308P; EMD Millipore), HRP-conjugated goat anti-rabbit IgG (AP307P; EMD Millipore), FITC-conjugated goat anti-mouse IgG (F0257; Sigma), and APC-conjugated goat anti-rabbit IgG. All antibodies were used at concentrations and conditions recommended by manufacturers.

Frey T.R., Akinyemi I.A., Burton E.M., Bhaduri-McIntosh S, & McIntosh M.T. (2021). An Ancestral Retrovirus Envelope Protein Regulates Persistent Gammaherpesvirus Lifecycles. Frontiers in Microbiology, 12, 708404.

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Andrographolide Modulation of miR-21

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Andrographolide (Andro, Sigma-Aldrich) was dissolved in dimethyl sulfoxide (DMSO). miRNA mimics of miR-21-5p and negative control RNA (NC) were obtained from RiboBio Co., Ltd. The following primary antibodies were used for immunoblotting: rabbit anti-TIMP3 (ab39184), obtained from Abcam (Cambridge, UK); and rabbit anti-GAPDH (#2118), obtained from Cell Signaling Technology, Inc. (Danvers, Massachusetts, USA).

Dai J., Lin Y., Duan Y., Li Z., Zhou D., Chen W., Wang L, & Zhang Q.Q. (2017). Andrographolide Inhibits Angiogenesis by Inhibiting the Mir-21-5p/TIMP3 Signaling Pathway. International Journal of Biological Sciences, 13(5), 660-668.

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DPPA Inhibition Modulates Cell Proliferation

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DPPA (BML-LP103) was obtained from Enzo Life Sciences, Inc. (NY, USA) and was dissolved in dimethyl sulfoxide (DMSO) at a concentration of 10mM with 15 minutes of sonication at room temperature. Cell Counting Kit-8 (CCK8) was obtained from Beyotime (China). Rabbit anti-GAPDH (#2118, diluted at 1:1000 for western blotting) was obtained from Cell Signaling Technology, Inc. (USA), while rabbit anti-Ki67 (GT210102) was obtained from Gene Tech (Shanghai) Co., Ltd. (China), and rabbit anti-CCNB1 (BA0766) and rabbit anti-CD34 (BA0532) were obtained from Boster (Wuhan) Co., Ltd. (China). CCNB1 siRNA was synthesized by RiboBio Co., Ltd. (China) according a previous report 26 (link).

Zhang Q.Q., Chen J., Zhou D.L., Duan Y.F., Qi C.L., Li J.C., He X.D., Zhang M., Yang Y.X, & Wang L. (2017). Dipalmitoylphosphatidic acid inhibits tumor growth in triple-negative breast cancer. International Journal of Biological Sciences, 13(4), 471-479.

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Investigating Cell Proliferation Regulation

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Andro (365645) and ammonium pyrrolidinedithiocarbamate (APDC; NF-κB inhibitor, P8765) were obtained from Sigma-Aldrich (St. Louis, MO, United States) and were dissolved in dimethyl sulfoxide (DMSO). Cell Counting Kit-8 (CCK-8) was purchased from Beyotime (Shanghai, China). Rabbit anti-Ki67 (ab15580) was purchased from Abcam (Cambridge, MA, United States), rabbit anti-GAPDH (2118) was purchased from Cell Signaling Technology, Inc. (Danvers, MA, United States), rabbit anti-PDCD4 (BM5208) and rabbit anti-EGN (endoglin, BA2227) were both from Boster (Wuhan, China), and rabbit anti-NF-κB p65 (abs131170) and rabbit anti-phospho-NF-κB p65 (Ser536, abs130624) were purchased from Absin (Shanghai, China). All the miRNA mimics and inhibitors and the negative control (NC) RNAs were purchased from RiboBio Co., Ltd. (Guangzhou, China). All the primers were synthesized by Sangon Biotech Co., Ltd. (Shanghai, China).

Li J., Huang L., He Z., Chen M., Ding Y., Yao Y., Duan Y., Zixuan L., Qi C., Zheng L., Li J., Zhang R., Li X., Dai J., Wang L, & Zhang Q.Q. (2021). Andrographolide Suppresses the Growth and Metastasis of Luminal-Like Breast Cancer by Inhibiting the NF-κB/miR-21-5p/PDCD4 Signaling Pathway. Frontiers in Cell and Developmental Biology, 9, 643525.

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Dipalmitoylphosphatidic Acid Cell Assay

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Dipalmitoylphosphatidic acid (BML‐LP103) was obtained from Enzo Life Sciences, Inc. (NY, USA) and was dissolved in DMSO at the concentration of 10 mmol/L according to our previously reported.11 Cell Counting Kit‐8 (CCK8) was obtained from Beyotime (Shanghai, China). Rabbit anti‐GAPDH (#2118) was obtained from Cell Signaling Technology, Inc. (USA), rabbit anti‐CD31 (ab28364) was obtained from Abcam (Cambridge, MA), rabbit anti‐FGF1 (BA0843) and rabbit anti‐HGF (BA0911) were obtained from Boster (Wuhan) Co., Ltd. (China), and rabbit anti‐CDP (sc‐13024) was obtained from Santa Cruz Biotechnology Inc. (Santa Cruz, CA, USA). All siRNAs were synthesized by RiboBio Co., Ltd. (Guangzhou, China).

Chen J., Zhou Z., Yao Y., Dai J., Zhou D., Wang L, & Zhang Q. (2018). Dipalmitoylphosphatidic acid inhibits breast cancer growth by suppressing angiogenesis via inhibition of the CUX1/FGF1/HGF signalling pathway. Journal of Cellular and Molecular Medicine, 22(10), 4760-4770.

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Immunoblotting and Immunofluorescence Protocols

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The following primary antibodies were used for immunoblotting: rabbit anti-GAPDH [2118] (1:5000) from Cell Signaling; rabbit anti-TOM20 [sc-11415] (1:1000) and mouse anti-HSP60 [sc-13115] (1:1000) from Santa Cruz Biotechnology (Dallas, TX, USA); anti-VDAC [ab-15895] (1:1000) from Abcam (Cambridge, UK); anti-TIM23 [611222] (1:1000) from BD Bioscience (San Jose, CA, USA). The following primary antibodies were used for immunofluorescence images: TOM20 [sc-11415] (1:100) from Santa Cruz Biotechnology, H3 [14269] (1:100), H3K9ac [9649] (1:100), and H3K9me3 [13969] (1:100) from Cell Signaling (Danvers, MA, USA).

Morganti C., Bonora M., Marchi S., Ferroni L., Gardin C., Wieckowski M.R., Giorgi C., Pinton P, & Zavan B. (2020). Citrate Mediates Crosstalk between Mitochondria and the Nucleus to Promote Human Mesenchymal Stem Cell In Vitro Osteogenesis. Cells, 9(4), 1034.

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