Dm2500p microscope

Manufactured by Leica

The DM2500P is a compound microscope designed for high-quality observation and documentation of samples. It features a sturdy, ergonomic design and a range of optical components to provide excellent image quality. The DM2500P is suitable for a variety of applications in research and educational settings.

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Lab products found in correlation

Geminisem 500, by Zeiss (1 mentions) Uv vis spectrophotometer, by PerkinElmer (1 mentions) Agilent 6890, by Agilent Technologies (1 mentions) Ar1000 rheometer, by TA Instruments (1 mentions) Av 400 spectrometer, by Bruker (1 mentions) Jsm 6301f, by JEOL (1 mentions) Jms 700, by JEOL (1 mentions) Advance drx 300, by Bruker (1 mentions) Biflex 3 spectrometer, by Bruker (1 mentions)

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DM2500P (48 citations)

5 protocols using dm2500p microscope

Characterization of Liquid Crystal-Functionalized Gold Nanoparticle Shells

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Leica DM2500P microscope in the transmission mode with a Leica 63x (NA = 0.80) objective and a Q-image Retiga camera is mounted on the microscope. Polarized optical microscopy and Fluorescence microscopy (reflection) are also carried out on the same microscope. For fluorescence imaging of encapsulated dye, Lumogen F Red (BASF) with a peak emission at 613 nm, we use a 515–560 nm band-pass filter with white-light mercury lamp illumination. Emission was detected using a 580 nm dichroic mirror and a 590 nm long pass filter. Leica DM2500P microscope is used for bright field, cross-polarized, fluorescence images of the NAMs.
Zeiss Gemini SEM 500 instrument operating at 3 kV and 10 kV was used to validate the size of individual NAMs or arrangement of the individual AuNPs in the shell wall. NAMs are extracted from the liquid crystal host and re-dispersed in chloroform after we formed shells in cooling the Liquid crystal-functionalized gold nanoparticle mixture from isotropic to nematic as described earlier. Extracted NAMs were pipetted on Indium Tin Oxide (ITO) glass slides and dried the sample at 80 °C environment for 5 hours. The NAMs are imaged after chloroform is completely evaporated.
Absorption spectral measurements for gold nanoparticles in chloroform are performed using a PerkinElmer UV/Vis spectrophotometer.

Quint M.T., Sarang S., Quint D.A., Keshavarz A., Stokes B.J., Subramaniam A.B., Huang K.C., Gopinathan A., Hirst L.S, & Ghosh S. (2017). Plasmon-actuated nano-assembled microshells. Scientific Reports, 7, 17788.

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Maceral and Mineral Analysis of Coal Bricks

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The maceral compositions
and minerals of the polished coal bricks
were obtained using a Leica DM2500P microscope. Maceral analysis was
performed using a point-counting technique. About 500 points were
counted per each sample. The yttrium aluminum garnet (0.912%) was
used as a reflectance standard. The vitrinite random reflectance (R_o) of coals was measured on a Zeiss Axioskop
40 photometer system following ASTM D2798-11a (2011).³¹The separated saturated and aromatic fractions were
measured by gas chromatography (GC, Agilent 6890) and gas chromatography-mass
spectrometry (GC-MS, Agilent 6890-MS5973). The temperature rising
procedures of chromatography were from 60 °C (hold time 5 min)
to 280 °C (hold time 15 min) at 4 °C/min. The mass spectrometry
was operated at the ionization energy of 70 eV, and the detected relative
molecular mass ranges from 50 to 650 Da.

Lu Q., Qin S., Xu F., Chang X, & Wang W. (2021). Maceral and Organic Geochemical Characteristics of the Late Permian Coals from Yueliangtian Mine, Guizhou, Southwestern China. ACS Omega, 6(4), 3149-3163.

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Pyrene-based Gelation Protocol

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The samples were prepared by mixing C3Pyr (8 mg.mL -1 ), the minimum amount of acceptor to observe gelation and TCE. These were subsequently heated till complete solubilisation and dropcasted on a glass slide. After evaporation of the solvent, micrographs were recorded with a Leica DM 2500P microscope.

Gainar A., Lai T.L., Oliveras-González C., Pop F., Raynal M., Isare B., Bouteiller L., Linares M., Canevet D., Avarvari N, & Sallé M. (2021). Tuning the Organogelating and Spectroscopic Properties of a C₃ -Symmetric Pyrene-Based Gelator through Charge Transfer. Chemistry (Weinheim an der Bergstrasse, Germany), 27(7).

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Emulsion Characterization via Light Scattering, Microscopy, and Rheometry

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The continuous phase of the emulsions was obtained by mixing 12 g of silicone based UV cross-linkable PDMS fluid together with 0.5 g of catalyzer, 10 mg of UV sensitizer and 50 mg of surfactant (Silube ® J208-812, later referred to as Silube or 2-octyl-1-dodecanol, later referred to as OH457). In order to obtain 30% vol. emulsions, 5.4 g of water phase containing 1.5 wt.% NaCl was introduced under mechanical stirring with a home-made helical device. The size distributions of emulsions were characterized both by static light scattering, optical microscopy and rheometry. Static light scattering experiments were performed in a Mastersizer S apparatus (Malvern) with dodecane as the continuous phase. In dodecane, emulsion droplets remain fully dispersed whatever the used surfactant. For optical measurements, a drop of the emulsion was placed between two glass slides and observed with a Leica DM 2500P microscope. Rheological properties of emulsions were characterized with a plate-plate geometry in an AR1000 rheometer (TA Instruments). We eliminate any slip at the plates by coating them with an appropriate sand paper.

Kovalenko A., Zimny K., Mascaro B., Brunet T, & Mondain-Monval O. (2016). Tailoring of the porous structure of soft emulsion-templated polymer materials. Soft matter, 12(23).

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Thorough Characterization of Chemical Compounds

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Starting materials were purchased and used without further purification. Thin-layer chromatography (TLC) was performed on aluminium plates coated with MerckSilica gel 60 F254. When necessary, silica gel 60 (35-70 mesh, SDS) was used to isolate the desired derivatives. C NMR spectra were recorded using deuterated solvents as internal references on a BRUKER Advance DRX 300 or a BRUKER AV400 spectrometer. Multiplicities are denoted as follows: s = singlet, d = doublet, t = triplet, m = multiplet, br = broad. The mass spectra were recorded on a Q-ToF spectrometer (accurate mass measurements were achieved using sodium formate as internal reference), a Jeol JMS 700 (high-resolution mass spectra (HRMS) or a Bruker Biflex III spectrometer (MALDI-TOF). The infrared absorption spectra were recorded on an FTIR BRUKER VERTEX 70. Optical microscopy was performed by employing a Leica DM 2500P microscope. SEM micrographs were recorded by scanning electron microscopy (SEM) with a JEOL JSM 6301F operating at tensions of 3 kV.

Belén Marco A., Gindre D., Iliopoulos K., Franco S., Andreu R., Canevet D, & Sallé M. (2018). (Super)gelators derived from push-pull chromophores: synthesis, gelling properties and second harmonic generation. Organic & biomolecular chemistry, 16(14).

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