Rosa26 lsl zsgreen

C57BL6/J, Rosa26-LSL-ZsGreen [20 (link)], Rosa26-LSL-Tdtomato [20 (link)], and TCRα^-/- [21 (link)] mice were purchased from the Jackson laboratory. CD4Cre mice [22 (link)] were purchased from Taconic Inc. Foxn1Cre mice [23 (link)] were kindly provided by Dr. Nancy Manley, University of Georgia. The mice were housed in a pathogen-free facility and were bred as described in the Results section. Mice were euthanized by CO2 followed by organ removal. Total 40 mice (18 male and 22 female mice) were used for experiments.

All mice were 8–16 weeks of age and maintained on a C57BL/6 background. Prrx1-cre (stock # 005584) recombining in bone marrow mesenchymal cells of the long bones, Lepr^cre (stock # 008320) recombining in bone marrow mesenchymal stromal cells, and Rosa26^LSL-ZsGreen (stock # 007906) mice were obtained from the Jackson Laboratory. Col1a1-cre mice (with 2.3 kb promoter), recombining in mature osteoblasts, were described previously (Liu et al., 2004 (link)). The generation of Thpo^gfp, Thpo^creER, and Thpo^fl mice was described previously (Decker et al., 2018 (link)). Thpo^gfp is a null allele of Thpo. Thpo^creER knockin mice allows tracing of cells that translate THPO protein. All mice were housed in specific pathogen-free, Association for the Assessment and Accreditation of Laboratory Animal Care (AAALAC)-approved facilities at the Columbia University Medical Center. All protocols were approved by the Institute Animal Care and Use Committee of Columbia University.