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Apc cy7 mouse anti human cd45 mab

Manufactured by BD

The APC‐Cy7 mouse anti‐human CD45 mAb is a monoclonal antibody conjugated with APC‐Cy7 fluorochrome. It is designed to detect the CD45 protein, which is a common leukocyte antigen expressed on the surface of various human blood cells.

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2 protocols using apc cy7 mouse anti human cd45 mab

1

Monocyte Expression Profiling in MPE and Blood

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The expression markers on monocytes from MPE and blood were determined by flow cytometry as previously described.21 The expression markers on monocytes from MPE and blood were determined by flow cytometry after surface staining with APC‐Cy7 mouse anti‐human CD45 mAb, FITC mouse anti‐human CD14 mAb, and PE‐Cy7 mouse anti‐human CD16 mAb (BD Biosciences). Flow cytometry was performed on a BD FACS CantoII flow cytometer using Flowjow 10.0 software.
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2

Monocyte Subsets Profiling in Pleural Effusion

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The expression markers on monocytes from pleural effusion and blood were determined by flow cytometry after surface staining with APC‐Cy7 mouse anti‐human CD45 mAb, FITC mouse anti‐human CD14 mAb, and PE‐Cy7 mouse anti‐human CD16 mAb (BD Biosciences). Different monocytes subsets were identified within the SSC‐Ahi CD45+ cells and gated for the CD14++CD16 classical, CD14++CD16+ intermediate and CD14+CD16++ nonclassical subsets. Flow cytometry was performed on a BD FACS CantoIIflow cytometer and analyzed by Flowjo 10.0.
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