Liver lipid accumulation was confirmed by Modified Oil Red O stain kit (Catalog No. G1261, Solarbio, Beijing, China) according to the manufacturer’s instructions. In brief, frozen slices of liver (6–10 μm) were fixed in 10% formaldehyde for 10 min, and then washed with 60% isopropanol for 20–30 s. Liver tissue was stained in Modified Oil Red O solution for 10–15 min. After staining, the slices were washed with 60% isopropanol and then with H2O. Images were obtained using standard methods, imaged with a Leica Aperio VERSA 8 microscope, and then analyzed with Image J (1.48v, Bethesda, MD).
Modified oil red o stain kit
Manufactured by Solarbio
Sourced in China
The Modified Oil Red O stain kit is a laboratory tool used for the detection and visualization of neutral lipids and lipid droplets in cells and tissues. It provides a standardized and reliable method for lipid staining.
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3 protocols using modified oil red o stain kit
1
Quantifying Liver Lipid Accumulation
2
Lipid Visualization in Liver and Adipose
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For Oil-red O staining, liver tissues were embedded in optimal cutting temperature compound before sectioning at -20 ˚C and cut into 10-µm thick sections. The sections were stained using a Modified Oil Red O Stain Kit (G1261, Solarbio). Adipose tissues from mice in each group were fixed in 4% paraformaldehyde for 24 h at 4 °C and then embedded in paraffin. The 5 μm thick sections of adipose tissues were obtained and then stained with hematoxylin-eosin (HE). Images were observed using a Nikon E200 light microscope.
3
Liver Lipid Accumulation Visualization
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Liver lipid accumulation was confirmed by Modified Oil Red O stain kit (Solarbio, G1261) according to the manufacturer's instructions. In brief, frozen slices of liver (6-10 μm) were fixed in 10% formaldehyde in PBS for 10 min, then washed with 60% isopropanol for 20-30 s. Liver tissue was stained in Modified Oil Red O solution for 10-15 min. After staining, the slices were washed with 60% isopropanol and then with H 2 O. Images were obtained using standard methods and imaged with a Leica Aperio VERSA 8 microscope, then analysed with Image J (Media Cybernetics).
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