Samples of approximately 1 mL broth were quenched using cold steel beads in a syringe, as described by Mashego et al. [18 (link)], and rapidly filtered using 0.45 μm disc filters (Millipore). 80 µL of filtrate were mixed with 20 µL of internal standard (500 µM 15N-NH4Cl) and derivatized according to the protocol used for ammonium quantification by LC-MS.
0.45 μm disc filters
Manufactured by Merck Group
The 0.45 μm disc filters are laboratory equipment designed for the filtration of liquids. They feature a pore size of 0.45 micrometers, which allows the effective removal of particles, microorganisms, and other contaminants from various liquid samples.
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Lab products found in correlation
2 protocols using 0.45 μm disc filters
1
Rapid Extraction and Quantification of Metabolites
2
Aerobic Nitrogen-Limited Chemostat Sampling
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For aerobic nitrogen limited chemostats, samples of approx. 2 mL were quenched using cold steel beads [24 (link)], and filtered using 0.45 μm disc filters (Milipore). Samples for residual ammonium determination were prepared by mixing 80 μL of sample with 20 μL of internal standard (500 μmol/L 15N-NH4Cl) and quantified according to [25 (link)]. All samples were stored at -80 °C until further analysis.
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