William s e glutamax 1 medium

Manufactured by Thermo Fisher Scientific

Sourced in Germany

William's E + GlutaMAX-I medium is a cell culture medium formulated for the maintenance and growth of various cell types. It contains the necessary nutrients, amino acids, and supplements to support cell proliferation and viability.

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Lab products found in correlation

Penicillin streptomycin, by Thermo Fisher Scientific (2 mentions) L glutamine, by Thermo Fisher Scientific (2 mentions) Fetal bovine serum (fbs), by Thermo Fisher Scientific (2 mentions) Liberase, by Roche (2 mentions) Rmil 6, by Thermo Fisher Scientific (1 mentions) Percoll gradient, by GE Healthcare (1 mentions) Rmil 22, by Thermo Fisher Scientific (1 mentions)

Spelling variants of this product

GlutaMAX-I (877 citations) William’s E medium (265 citations) Williams’ Medium E (101 citations) GlutaMAX medium (64 citations) William’s medium (24 citations) William’s E (21 citations) Williams’ Medium E-GlutaMAX (9 citations) William E medium (9 citations) GlutaMAX-I medium (7 citations) Williams’ medium E glutamax‐I (2 citations)

2 protocols using william s e glutamax 1 medium

Isolation and Culture of Primary Hepatocytes

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For the isolation of primary hepatocytes, the liver was digested with Liberase (Roche Diagnostics) and was then gently disrupted to free residual cells. Single-cell suspension was filtered through a 100-μm cell strainer and the cells were allowed to settle by gravity for 20 min. Subsequently, parenchymal cells were separated by 10 min centrifugation in a 90% Percoll gradient (GE Healthcare). For primary hepatocyte culture, William’s E + GlutaMAX-I medium (Life Technologies, Karlsruhe, Germany) was supplemented with 10% FBS (Life Technologies), 1% penicillin/streptomycin (Life Technologies) and 1% L-glutamine (Life Technologies). The cells were incubated overnight at 37°C, 40% O₂. Subsequently, the hepatocytes were washed and incubated with 100 ng/mL rmIL-22 or rmIL-6 for 15 min (eBioscience; R&D Systems; Peprotech, respectively). Finally, the cells were harvested and the RNA was extracted.

Giannou A.D., Kempski J., Shiri A.M., Lücke J., Zhang T., Zhao L., Zazara D.E., Cortesi F., Riecken K., Amezcua Vesely M.C., Low J.S., Xu H., Kaffe E., Garcia-Perez L., Agalioti T., Yamada Y., Jungraithmayr W., Zigmond E., Karstens K.F., Steglich B., Wagner J., Konczalla L., Carambia A., Schulze K., von Felden J., May P., Briukhovetska D., Bedke T., Brockmann L., Starzonek S., Lange T., Koch C., Riethdorf S., Pelczar P., Böttcher M., Sabihi M., Huber F.J., Reeh M., Grass J.K., Wahib R., Seese H., Stüben B.O., Fard-Aghaie M., Duprée A., Scognamiglio P., Plitzko G., Meiners J., Soukou S., Wittek A., Manthey C., Maroulis I.C., Arck P.C., Perez D., Gao B., Zarogiannis S.G., Strowig T., Pasqualini R., Arap W., Gosálvez J.S., Kobold S., Prinz I., Guse A.H., Tachezy M., Ghadban T., Heumann A., Li J., Melling N., Mann O., Izbicki J.R., Pantel K., Schumacher U., Lohse A.W., Flavell R.A., Gagliani N, & Huber S. (2023). Tissue resident iNKT17 cells facilitate cancer cell extravasation in liver metastasis via interleukin-22. Immunity, 56(1), 125-142.e12.

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Isolation and Culture of Primary Hepatocytes

Cited 1 time

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The isolation was carried out according to standard protocols [49 (link)]. First, the liver was digested by perfusion with Liberase (Roche Diagnostics, Basel, Switzerland) and was then gently disrupted to free residual cells. The single-cell suspension was filtered through a 100 μm cell strainer and the cells were allowed to settle by gravity for 20 min. Subsequently, parenchymal cells were separated by 10 min centrifugation in a 90% Percoll gradient (GE Healthcare, Chicago, IL, USA). For primary hepatocyte culture, William’s E + GlutaMAX -I medium (Life Technologies, Karlsruhe, Germany) was supplemented with 10% FBS (Life Technologies, Karlsruhe, Germany), 1% penicillin/streptomycin (Life Technologies, Karlsruhe, Germany), and 1% L-glutamine (Life Technologies, Karlsruhe, Germany). Cells were incubated overnight at 37 °C with 40% O₂. On the next day, the hepatocytes were washed and incubated with 1 ng/mL recombinant mIL-22 (eBioscience, San Diego, CA, USA) for 15 min.

Giannou A.D., Lücke J., Kleinschmidt D., Shiri A.M., Steglich B., Nawrocki M., Zhang T., Zazara D.E., Kempski J., Zhao L., Giannou O., Agalioti T., Brockmann L., Bertram F., Sabihi M., Böttcher M., Ewald F., Schulze K., von Felden J., Machicote A., Maroulis I.C., Arck P.C., Grass J.K., Mercanoglu B., Reeh M., Wolter S., Tachezy M., Seese H., Theodorakopoulou M., Lykoudis P.M., Heumann A., Uzunoglu F.G., Ghadban T., Mann O., Izbicki J.R., Li J., Duprée A., Melling N., Gagliani N, & Huber S. (2022). A Critical Role of the IL-22–IL-22 Binding Protein Axis in Hepatocellular Carcinoma. Cancers, 14(24), 6019.

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