Hrp conjugated anti human igg fab 2 goat polyclonal antibody

The following human recombinant proteins were used: human chimeric SARS-CoV-2 (2019-nCoV) Spike-RBD/Fc protein was purchased from Sino Biological (Eschborn, Germany). Human chimeric SARS-CoV-2 BA.1 Omicron (B1.1.529) Spike-RBD/Fc protein was purchased from Sino Biological. Human chimeric SARS-CoV-2 Omicron BA.4/BA.5 Spike-RBD/His protein was purchased from R&D Systems (Minneapolis, MN, USA). Human chimeric ACE-2/Fc protein was purchased from GenScript (Piscataway, NJ, USA). Human Recombinant IgG1 Fc protein was from Abcam (Cambridge, UK).
The following antibodies were used: Tixagevimab and Cilgavimab, two human Fc-modified IgG1 derived from B cells from two individuals who had recovered from SARS-CoV-2 infection and developed by AstraZeneca (Samsung Biologics, Incheon, Republic of Korea). D3 mAb is a fully human IgG4 mAb isolated by phage display, which was expressed and purified as previously described [11 ].
Human SARS-CoV-2 Spike protein (RBD) polyclonal antibody was purchased from Invitrogen (Rockford, IL, USA). HRP-conjugated anti-human IgG (Fab’)2 goat polyclonal antibody from Abcam (Cambridge, UK) was used for the detection of primary mAbs.

The following recombinant proteins were used: Human PD-L1/Fc, human PD-1/Fc and human LAG-3/Fc protein (all from Bio-Thecne R&D Systems, Inc., Minneapolis, MN, USA); Human LAG-3/His-GST and Human HLA class II histocompatibility antigen, DRA (from Cusabio Technology LLC, Houston, TX, USA); and Staphylococcal enterotoxin B (SEB), a toxin produced by the bacterium Staphylococcus aureus and used as stimuli for the activation of lymphocytes (Sigma, S4881 St. Louis, MO, USA).
The following antibodies were used: HRP-conjugated antihuman IgG (Fab’)2 goat polyclonal antibody (Abcam, Milan, Italy); anti-His-HRP-conjugated antibody (Proteintech, Deansgate, Germany); HRP-conjugated Streptavidin (Biorad, Milan, Italy); antihuman p44/42 MAPK (T202/Y204), antihuman Cleaved Caspase-3; anti-phospho-Sapk/Jnk (T183/Y185) rabbit polyclonal antibodies (all from Cell Signaling, Danvers, MA, USA); antivinculin monoclonal antibody (all from Santa Cruz Biotechnology, Inc. Dallas, TX, USA); and HRP-conjugate anti-mouse IgG and antirabbit secondary antibodies (all from Sigma, USA). PD-L1_1 (anti-PD-L1), PD-1_1 (anti-PD-1), LAG-3_1 (anti-LAG-3). Human IgG control (unrelated) monoclonal antibodies were produced in our laboratory, as previously described [32 (link)].