Tert butyl 4 hydroxy toluene

All chemicals used in the present study were of analytical grade. Compounds such as the Folin-Ciocalteu reagent, quercetin, kanamycin, vancomycin, α-tocopherol, tert-butyl-4-hydroxy toluene (BHT), 2,2-diphenyl-1-picryl-hydrazyl-hydrate (DPPH), 2,2′-azino-bis-3-ethylbenzthiazoline-6-sulphonic acid, 2,4,6-tri(2-pyridyl)-s-triazine (TPTZ), and gallic acid were obtained from Sigma-Aldrich Chemical Co. (St. Louis, MO, USA). Hydrogen peroxide (H₂O₂) was obtained from Sigma-Aldrich (Seoul, Korea), and nitric acid (HNO₃) was purchased from Showa Chemical Industry Co. Ltd. (Tokyo, Japan). Ultrapure distilled water, which was used in the analysis, was purchased from the Zeneer power 1 system (Human Corporation, Seoul, Korea). The 19 multielement standards used in the analysis (micro- and macro-elements) were purchased from Perkin Elmer (Seoul, Korea). The multielement stock solutions were obtained from Quality Control Standard 26, (Inorganic Ventures, Christiansburg, VA, USA). All analytical equipment required for sample preparation and mineral quantification were cleaned with HNO₃ (2%, v/v) and rinsed thrice with ultrapure water.

All solvents used in this study were of analytical grade. Methanol was obtained from Baker (Phillipsburg, NJ, USA). Sodium carbonate, gallic acid, potassium acetate, aluminum nitrate, and aluminum chloride were obtained from Sigma-Aldrich Chemical Co. (St. Louis, MO, USA). 1,1-Diphenyl-2-picrylhydrazyl (DPPH), 2,2-azino-bis-3-ethylbenzthiazoline-6-sulphonic acid (ABTS⁺), Folin–Ciocalteu reagent, quercetin, and tert-butyl-4-hydroxy toluene (BHT) were obtained from Sigma Chemical Co. (St. Louis, MO, USA). Ultrapure distilled water was obtained from the Zeneer power 1 system (Human Corporation, Seoul, Korea).