Goat polyclonal antiserum against pgc 1α and nrf1

Manufactured by Santa Cruz Biotechnology

Goat polyclonal antiserum against PGC-1α and NRF1 is a laboratory product developed by Santa Cruz Biotechnology. It contains antibodies produced in goats that recognize the PGC-1α and NRF1 proteins.

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Lab products found in correlation

Neuronal nuclei (neun), by Merck Group (2 mentions) Ax 51 epifluorescence microscope, by Olympus (2 mentions) Neuronal nuclei (neun), by Olympus (2 mentions) Goat anti mouse igg conjugated with alexa fluor 568, by Thermo Fisher Scientific (1 mentions) Goat anti rabbit igg conjugated with alexa fluor 488, by Thermo Fisher Scientific (1 mentions) Alexa fluor 568, by Thermo Fisher Scientific (1 mentions) Goat anti mouse igg, by Thermo Fisher Scientific (1 mentions) Goat anti rabbit igg, by Thermo Fisher Scientific (1 mentions) Alexa fluor 488, by Thermo Fisher Scientific (1 mentions)

Spelling variants of this product

PGC-1α (105 citations)

2 protocols using goat polyclonal antiserum against pgc 1α and nrf1

Dual Immunofluorescence Detection of PGC-1α and NeuN

Cited 1 time

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Double immunofluorescence staining [5 (link),7 (link),8 (link),43 (link),56 (link)] was carried out using a goat polyclonal antiserum against PGC-1α and NRF1 (Santa Cruz Biotechnology) and a mouse monoclonal antiserum against a specific neuronal marker, neuron-specific nuclear protein (NeuN; Chemicon). The secondary antisera included goat anti-rabbit IgG conjugated with AlexaFluor 488 and goat anti-mouse IgG conjugated with Alexa Fluor 568 (Molecular Probes, Eugene, OR, USA). Sections were viewed under an Olympus AX-51 epifluorescence microscope (Olympus, Kyoto, Japan); immunoreactivity for NeuN exhibited red fluorescence and PGC-1α manifested green fluorescence.

Chuang Y.C., Chen S.D., Hsu C.Y., Chen S.F., Chen N.C, & Jou S.B. (2019). Resveratrol Promotes Mitochondrial Biogenesis and Protects against Seizure-Induced Neuronal Cell Damage in the Hippocampus Following Status Epilepticus by Activation of the PGC-1α Signaling Pathway. International Journal of Molecular Sciences, 20(4), 998.

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Double Immunofluorescence Staining for PGC-1α and NRF1

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According to our previous studies [15 (link),74 (link)], double immunofluorescence staining was accomplished by using a goat polyclonal antiserum against PGC-1α and NRF1 (Santa Cruz Biotechnology), and a mouse monoclonal antiserum against neuron-specific nuclear protein (a specific neuronal marker, NeuN; Chemicon). The secondary antisera contained a goat anti-rabbit IgG conjugated with AlexaFluor 488 and a goat anti-mouse IgG conjugated with Alexa Fluor 568 (Molecular Probes, Eugene, OR, USA). Tissue sections were examined under an Olympus AX-51 epifluorescence microscope (Olympus, Kyoto, Japan); immunoreactivity for NeuN exhibited red fluorescence, and PGC-1α and NRF1 manifested green fluorescence.

Chuang Y.C., Chen S.D., Jou S.B., Lin T.K., Chen S.F., Chen N.C, & Hsu C.Y. (2019). Sirtuin 1 Regulates Mitochondrial Biogenesis and Provides an Endogenous Neuroprotective Mechanism Against Seizure-Induced Neuronal Cell Death in the Hippocampus Following Status Epilepticus. International Journal of Molecular Sciences, 20(14), 3588.

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