Tissues were ground in liquid nitrogen and suspended in pre-cooled acetone (− 20 °C) containing 10% (w/v) trichloroacetic acid. After vigorously mixing the solutions, the proteins were precipitated for 16 h at − 20 °C and then collected by centrifuging at 16,000×g (Beckman Allegra 22R) for 30 min at 4 °C. The pelleted proteins were washed three times with pre-cooled acetone. After a lyophilization, the pellets were dissolved in extraction buffer [8 M urea, 50 mM triethylammonium bicarbonate (TEAB), pH 8.5, 1% SDS, and protease inhibitor cocktail (Roche, Indianapolis, USA)]. The soluble protein extracts were collected by centrifuging at 16,000×g for 20 min at 4 °C, after which the protein concentrations were determined with the BCA assay kit (Pierce, Rockford, IL, USA).
Allegra 22r
Manufactured by Beckman Coulter
The Allegra 22R is a high-performance refrigerated benchtop centrifuge designed for a wide range of laboratory applications. It features a compact and ergonomic design, accommodating a variety of sample sizes and types. The Allegra 22R provides consistent, reliable performance with precise temperature control and speed capabilities.
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Lab products found in correlation
4 protocols using allegra 22r
1
Protein Extraction from Frozen Tissues
2
Protein Extraction and Quantification
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Tissues were ground in liquid nitrogen and suspended in pre-cooled acetone (-20°C) containing 10% (w/v) trichloroacetic acid. After vigorously mixing the solutions, the proteins were precipitated for 16 h at -20°C and then collected by centrifuging at 16,000 × g (Beckman Allegra 22R) for 30 min at 4°C. The pelleted proteins were washed three times with pre-cooled acetone. After a lyophilization, the pellets were dissolved in extraction buffer [8 M urea, 50 mM triethylammonium bicarbonate (TEAB), pH 8.5, 1% SDS, and protease inhibitor cocktail (Roche, Indianapolis, USA)]. The soluble protein extracts were collected by centrifuging at 16,000 × g for 20 min at 4°C, after which the protein concentrations were determined with the BCA assay kit (Pierce, Rockford, IL, USA).
3
Protein Extraction and Quantification
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Tissues were ground in liquid nitrogen and suspended in pre-cooled acetone (-20°C) containing 10% (w/v) trichloroacetic acid. After vigorously mixing the solutions, the proteins were precipitated for 16 h at -20°C and then collected by centrifuging at 16,000 × g (Beckman Allegra 22R) for 30 min at 4°C. The pelleted proteins were washed three times with pre-cooled acetone. After a lyophilization, the pellets were dissolved in extraction buffer [8 M urea, 50 mM triethylammonium bicarbonate (TEAB), pH 8.5, 1% SDS, and protease inhibitor cocktail (Roche, Indianapolis, USA)]. The soluble protein extracts were collected by centrifuging at 16,000 × g for 20 min at 4°C, after which the protein concentrations were determined with the BCA assay kit (Pierce, Rockford, IL, USA).
4
Protein Extraction from Frozen Tissues
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Tissues were ground in liquid nitrogen and suspended in pre-cooled acetone (-20 °C) containing 10% (w/v) trichloroacetic acid. After vigorously mixing the solutions, the proteins were precipitated for 16 h at -20 °C and then collected by centrifuging at 16,000 × g (Beckman Allegra 22R) for 30 min at 4 °C. The pelleted proteins were washed three times with pre-cooled acetone. After a lyophilization, the pellets were dissolved in extraction buffer [8 M urea, 50 mM triethylammonium bicarbonate (TEAB), pH 8.5, 1% SDS, and protease inhibitor cocktail (Roche, Indianapolis, USA)]. The soluble protein extracts were collected by centrifuging at 16,000 × g for 20 min at 4 °C, after which the protein concentrations were determined with the BCA assay kit (Pierce, Rockford, IL, USA).
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