Anti rabbit phosho egfr conjugated with phycoerythrin

To assess the phosphorylation of c-Met and EGFR in vivo, immunocompetent mice were infected with the various C. albicans strains as described above. After 1-d of infection, the mice were sacrificed, and the tongues were excised, snap frozen, and embedded into OTC. Thin sections were prepared and transferred to glass slides. The samples were air dried, fixed in 100% methanol, rinsed, and then blocked with 5% goat serum in PBS. The slides were incubated with a rabbit anti-phospho-c-MET antibody (Tyr1003, #MBS9600900, My Biosource Inc.) or control rabbit IgG (#026102, Invitrogen) followed an Alexa Fluor 568conjugated goat anti-rabbit antibody. The C. albicans cells were labeled with an anti-Candida antibody conjugated with Alexa Fluor 488 and the nuclei were labeled with DAPI. Phosphorylated EGFR was detected similarly, except that the slides were incubated with an anti-rabbit phosho-EGFR conjugated with phycoerythrin (Tyr1068, #14565, Cell Signaling Technologies) and control slides were stained with rabbit IgG conjugated with phycoerthrin (#5742, Cell Signaling Technologies). The slides were imaged by confocal microscopy, and z-stacks were combined using LAS X software.

To assess the phosphorylation of c-Met and EGFR in vivo, immunocompetent mice were infected with the various C. albicans strains as described above. After 1-d of infection, the mice were sacrificed, and the tongues were excised, snap frozen, and embedded into OTC. Thin sections were prepared and transferred to glass slides. The samples were air dried, fixed in 100% methanol, rinsed, and then blocked with 5% goat serum in PBS. The slides were incubated with a rabbit anti-phospho-c-MET antibody (Tyr1003, #MBS9600900, My Biosource Inc.) or control rabbit IgG (#026102, Invitrogen) followed an Alexa Fluor 568-conjugated goat anti-rabbit antibody. The C. albicans cells were labeled with an anti-Candida antibody conjugated with Alexa Fluor 488 and the nuclei were labeled with DAPI. Phosphorylated EGFR was detected similarly, except that the slides were incubated with an anti-rabbit phosho-EGFR conjugated with phycoerythrin (Tyr1068, #14565, Cell Signaling Technologies) and control slides were stained with rabbit IgG conjugated with phycoerthrin (#5742, Cell Signaling Technologies). The slides were imaged by confocal microscopy, and z-stacks were combined using LAS X software.