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Rabbit anti upf1 antibody

Manufactured by Merck Group
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Sourced in United States

Rabbit anti-UPF1 antibody is a laboratory reagent used for the detection and analysis of the UPF1 protein in biological samples. UPF1 is an important protein involved in the nonsense-mediated mRNA decay pathway. The antibody can be used in various applications such as Western blotting, immunoprecipitation, and immunohistochemistry to study the expression and localization of UPF1.

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Lab products found in correlation

Ez magna rip kit, by Merck Group (2 mentions) Control rabbit igg, by Merck Group (2 mentions)

Spelling variants of this product

Rabbit anti- (2 citations) Rabbit anti-phospho-UPF1 antibody (2 citations)

2 protocols using rabbit anti upf1 antibody

1

Profiling UPF1-Bound RNAs Using RIP

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RIP was performed using a EZ-Magna RIP kit (Millipore, USA) according to the manufacturer’s instructions. In brief, Hs578t or MCF-7 cells were lysed in the complete RIP lysis buffer and then the lysates were incubated with beads-antibody in RIP buffer. Rabbit anti-UPF1 antibody and rabbit IgG control (Millipore, USA) were used. Finally, the co-precipitated RNAs were extracted and detected by qRT-PCR.
Liu S., Chen W., Hu H., Zhang T., Wu T., Li X., Li Y., Kong Q., Lu H, & Lu Z. (2021). Long noncoding RNA PVT1 promotes breast cancer proliferation and metastasis by binding miR-128-3p and UPF1. Breast Cancer Research : BCR, 23, 115.
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2

UPF1 RNA Immunoprecipitation Protocol

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RIP was performed using a EZ-Magna RIP kit (Millipore, USA) according to the manufacturer's instructions. In brief, Hs578t or MCF-7 cells were lysed in the complete RIP lysis buffer and then the lysates were incubated with beads-antibody in RIP buffer. Rabbit anti-UPF1 antibody and rabbit IgG control (Millipore, USA) were used. Finally, the co-precipitated RNAs were extracted and detected by qRT-PCR.
Liu S., Chen W., Hu H., Zhang T., Wu T., Li X., Li Y., Kong Q., Lu H., & Lu Z. (2021). Long Noncoding RNA PVT1 Promotes Breast Cancer Proliferation and Metastasis by Binding mIR-128-3p and UPF1.
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